Assessment of short-term spatio-temporal variability in the structure of mesozooplankton communities integrating microscopy and multigene high-throughput sequencing
Assessment of short-term spatio-temporal variability in the structure of mesozooplankton communities integrating microscopy and multigene high-throughput sequencing
复制标题
结合显微镜和多基因高通量测序评估中型浮游生物群落结构的短期时空变异
DOI：
10.1016/j.ecss.2022.108038
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Mercado, Jesús M.
中科院分区：
文献类型：
--
作者：
Yebra, Lidia;García-Gómez, Candela;Valcárcel-Pérez, Nerea;Hernández de Rojas, Alma;Blanco-Bercial, Leocadio;Castro, M. Carmen;Gómez-Jakobsen, Francisco;Mercado, Jesús M.
在组成和结构的变化的中型浮游动物群落在海湾的马拉加（西南地中海）的特点是在26小时的周期，采用综合分类的方法。我们结合了微生物的显微鉴定，以及线粒体DNA COI和核糖体RNA 18S的V9高变区基因的元巴编码。通过显微镜获得的丰富度和多样性高于COI测量的，因为COI没有检测到一些门。然而，COI允许在显微镜下以较高的分类等级对几个分类群进行物种水平的鉴定。18S比COI和显微镜检测到更广泛的分类群，但分类分辨率较低。沿海夜间和货架日浮游动物群落结构之间的差异进行了检测显微镜和metabarcoding。这两种方法的结合增加了已知的桡足类物种在西南地中海的9%。提出了一种结合形态学和COI元编码的综合方法，以进一步促进中型浮游动物生物多样性研究。
Variability in composition and structure of the mesozooplankton communities in the Bay of Malaga (SW Mediterranean) were characterized during a 26 h cycle using an integrative taxonomic approach. We combined microscopic identification of organisms, with metabarcoding for the genes of the mitochondrial DNA COI and the V9 hypervariable region of the ribosomal RNA 18S. Richness and diversity obtained by microscopy were higher than those measured with COI, as COI did not detect some phyla. COI however allowed for the identification to species level of several taxa that were left at higher taxonomic rank under the microscope. 18S detected a wider range of taxa than COI and microscopy, although with lower taxonomic resolution. Differences between coastal-night and shelf-day zooplankton communities structure were detected by both microscopy and metabarcoding. The combination of these two approaches increased the known copepod species in the SW Mediterranean Sea by 9%. An integrative approach combining morphology and COI metabarcoding is proposed to further facilitate mesozooplankton biodiversity studies.
登录
查看更多内容
中上层的障碍：欧洲水域中的 Calanus helgolandicus 和 C. euxinus 的种群结构
DOI：
--
发表时间：
2011
期刊：
影响因子：
--
作者：
L. Yebra;D. Bonnet;R. Harris;P. Lindeque;K. Peijnenburg
通讯作者：
K. Peijnenburg
影响因子：
3.7
作者：
Hirai, Junya;Katakura, Seiji;Nagai, Satoshi
通讯作者：
Nagai, Satoshi
影响因子：
3.7
作者：
Blanco-Bercial, Leocadio
通讯作者：
Blanco-Bercial, Leocadio
DOI：
10.1098/rstb.2019.0446
发表时间：
2020-12-21
期刊：
Philosophical transactions of the Royal Society of London. Series B, Biological sciences
影响因子：
--
作者：
Laakmann S;Blanco-Bercial L;Cornils A
通讯作者：
Cornils A
1992-2020年西南地中海浮游动物群落的时空变化：与环境驱动因素的联系
影响因子：
4.1
作者：
L. Yebra;Marta Puerto;N. VALCÁRCEL;S. Putzeys;F. Gómez;C. García;J. Mercado
通讯作者：
J. Mercado