A modified elliptical formula to estimate kidney collagen content in a model of chronic kidney disease.
A modified elliptical formula to estimate kidney collagen content in a model of chronic kidney disease.
复制标题
一种修饰的椭圆形公式，以估计慢性肾脏疾病模型中的肾脏胶原蛋白含量。
DOI：
10.1371/journal.pone.0190815
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Narayan P
中科院分区：
文献类型：
--
作者：
Nieto JA;Zhu J;Duan B;Li J;Zhou P;Paka L;Yamin MA;Goldberg ID;Narayan P
慢性肾脏疾病（CKD）的瘢痕形成或肾间质胶原沉积的程度只能通过高侵入性、痛苦且有时危险的组织活检来确定。有趣的是，虽然ckd相关的肾脏大小异常通常可以通过超声显示，但不仅目前使用的椭球公式低估了肾脏的真实大小，而且计算出的肾脏大小并不能告知小管间质胶原含量。我们利用健康小鼠和肾病小鼠的冠状肾切片，建立了估算肾实质面积的新公式。该技术将肾脏视为一个以长轴(a)为极距的椭圆，将短轴(b)延伸至肾盂以获得一个新的短轴(b)。计算的肾实质面积与真实或测量的面积非常相似。生物化学测定的肾脏胶原含量与计算的肾实质面积呈正相关。小管间质胶原的小天狼星红染色也与计算的肾实质面积相关。肾瘢痕的范围，即肾间质胶原含量，现在可以通过两轴测量来计算，这可以很容易地通过该器官的无创成像来完成。
The extent of scarring or renal interstitial collagen deposition in chronic kidney disease (CKD) can only be ascertained by highly invasive, painful and sometimes risky, tissue biopsy. Interestingly, while CKD-related abnormalities in kidney size can often be visualized using ultrasound, not only does the ellipsoid formula used today underestimate true renal size, but the calculated renal size does not inform tubulointerstitial collagen content. We used coronal kidney sections from healthy mice and mice with kidney disease to develop a new formula for estimating renal parenchymal area. While treating the kidney as an ellipse with the major axis (a) the polar distance, this technique involves extending the minor axis (b) into the renal pelvis to obtain a new minor axis, be. The calculated renal parenchymal area is remarkably similar to the true or measured area. Biochemically determined kidney collagen content revealed a strong and positive correlation with the calculated renal parenchymal area. Picrosirius red staining for tubulointerstitial collagen also correlated with calculated renal parenchymal area. The extent of renal scarring, i.e. kidney interstitial collagen content, can now be computed by making just two axial measurements which can easily be accomplished via noninvasive imaging of this organ.
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