Dietary influences on the Dahl SS rat gut microbiota and its effects on salt-sensitive hypertension and renal damage.
Dietary influences on the Dahl SS rat gut microbiota and its effects on salt-sensitive hypertension and renal damage.
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DOI：
10.1111/apha.13662
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发表时间：
2021-08
影响因子：
6.3
通讯作者：
Mattson, David L.
中科院分区：
文献类型：
--
作者：
Abais-Battad, Justine M.;Saravia, Fatima L.;Lund, Hayley;Dasinger, John Henry;Fehrenbach, Daniel J.;Alsheikh, Ammar J.;Zemaj, Jeylan;Kirby, John R.;Mattson, David L.
我们以前的研究已经证明了饮食因素在测定Dahl盐敏感（SS）大鼠高血压中的重要性。由于肠道微生物群与高血压等慢性疾病有关，我们假设饮食改变会改变微生物群，从而介导盐敏感性高血压和肾脏疾病的发展。本研究使用来自威斯康星州医学院（SS/MCW）的SS大鼠，其维持在纯化的基于酪蛋白的饮食（0.4% NaCl AIN-76 A，染料）上，以及来自Charles River实验室（SS/CRL）的大鼠，其饲喂全谷物饮食（0.75% NaCl 5L 79，LabDiet）。使用粪便16 S rDNA测序来表型肠道微生物群。直接检查肠道微生物群的贡献，对SS/CRL大鼠施用SS/MCW粪便或媒介物（Vehl）与HS AIN-76 A饮食结合的粪便微生物群转移（FMT）实验。当喂食高盐（HS，4.0%NaCl AIN-76 A）时，SS/MCW大鼠表现出肾损伤和炎症，这在SS/CRL中显著减弱。肠道微生物群表型分析揭示了与疾病严重程度相关的不同特征。SS/MCW FMT使SS/CRL对HS的反应恶化，表现为白蛋白尿增加（67.4 ± 6.9 vs 113.7 ± 25.0 mg/d，Vehl vs FMT，P = 0.007）、动脉收缩压增加（158.6 ± 5.8 vs 177.8 ± 8.9 mmHg，Vehl vs FMT，P = 0.09）和肾T细胞浸润（1.9倍）。基于扩增子序列变体（ASV）的粪便16 S rDNA测序数据分析显示，FMT显著改变了分类群：丹毒丝菌科_2、戈登副杆菌、解乳链球菌、拟杆菌科_1、脱硫弧菌科_2、白色瘤胃球菌。这些数据表明，肠道微生物群的饮食调节直接有助于Dahl SS高血压和肾损伤的发展。
Our previous studies have demonstrated the importance of dietary factors in the determination of hypertension in Dahl salt-sensitive (SS) rats. Since the gut microbiota has been implicated in chronic diseases like hypertension, we hypothesized that dietary alterations shift the microbiota to mediate the development of salt-sensitive hypertension and renal disease. This study utilized SS rats from the Medical College of Wisconsin (SS/MCW) maintained on a purified, casein-based diet (0.4% NaCl AIN-76A, Dyets) and from Charles River Laboratories (SS/CRL) fed a whole grain diet (0.75% NaCl 5L79, LabDiet). Faecal 16S rDNA sequencing was used to phenotype the gut microbiota. Directly examining the contribution of the gut microbiota, SS/CRL rats were administered faecal microbiota transfer (FMT) experiments with either SS/MCW stool or vehicle (Vehl) in conjunction with the HS AIN-76A diet. SS/MCW rats exhibit renal damage and inflammation when fed high salt (HS, 4.0% NaCl AIN-76A), which is significantly attenuated in SS/CRL. Gut microbiota phenotyping revealed distinct profiles that correlate with disease severity. SS/MCW FMT worsened the SS/CRL response to HS, evidenced by increased albuminuria (67.4 ± 6.9 vs 113.7 ± 25.0 mg/day, Vehl vs FMT, P = .007), systolic arterial pressure (158.6 ± 5.8 vs 177.8 ± 8.9 mmHg, Vehl vs FMT, P = .09) and renal T-cell infiltration (1.9-fold). Amplicon sequence variant (ASV)-based analysis of faecal 16S rDNA sequencing data revealed taxa that significantly shifted with FMT: Erysipelotrichaceae_2, Parabacteroides gordonii, Streptococcus alactolyticus, Bacteroidales_1, Desulfovibrionaceae_2, Ruminococcus albus. These data demonstrate that dietary modulation of the gut microbiota directly contributes to the development of Dahl SS hypertension and renal injury.
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DOI：
10.1161/hypertensionaha.110.154302
发表时间：
2011-02
期刊：
Hypertension (Dallas, Tex. : 1979)
影响因子：
--
作者：
De Miguel C;Lund H;Mattson DL
通讯作者：
Mattson DL
影响因子：
11.1
作者：
Bahr SM;Weidemann BJ;Castro AN;Walsh JW;deLeon O;Burnett CM;Pearson NA;Murry DJ;Grobe JL;Kirby JR
通讯作者：
Kirby JR
DOI：
10.1161/hypertensionaha.114.04179
发表时间：
2015-02
期刊：
Hypertension (Dallas, Tex. : 1979)
影响因子：
--
作者：
Geurts AM;Mattson DL;Liu P;Cabacungan E;Skelton MM;Kurth TM;Yang C;Endres BT;Klotz J;Liang M;Cowley AW Jr
通讯作者：
Cowley AW Jr
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
--
作者：
Elliott, P;Stamler, J;Zhou, BF
通讯作者：
Zhou, BF