l-Theanine protects against excess dopamine-induced neurotoxicity in the presence of astrocytes.
l-Theanine protects against excess dopamine-induced neurotoxicity in the presence of astrocytes.
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DOI：
10.3164/jcbn.16-15
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发表时间：
2016-09
影响因子：
2.4
通讯作者：
Asanuma M
中科院分区：
文献类型：
--
作者：
Takeshima M;Miyazaki I;Murakami S;Kita T;Asanuma M
l-茶氨酸（γ-谷氨酰乙酰胺）是绿色茶的一种成分，被认为在大脑中具有调节和神经保护作用。本研究旨在通过细胞培养和动物实验来确定l-茶氨酸对过量多巴胺诱导的神经毒性的影响。将原代培养的中脑神经元或中脑神经元与纹状体星形胶质细胞的共培养物用l-茶氨酸预处理72 h，然后用过量多巴胺再处理24 h。评价多巴胺神经元的细胞活力和谷胱甘肽水平。过量多巴胺诱导的神经毒性显着衰减72小时预孵育与L-茶氨酸在神经元-星形胶质细胞共培养，但不是在神经元丰富的文化。暴露于l-茶氨酸增加了星形胶质细胞和神经胶质细胞条件培养基中谷胱甘肽的水平。来自l-茶氨酸预处理的纹状体星形胶质细胞的胶质细胞条件培养基减弱多巴胺诱导的中脑神经元的神经毒性和奎诺蛋白形成。此外，在体外无细胞谷胱甘肽合成系统中用L-茶氨酸替代L-谷氨酸产生谷胱甘肽样硫醇化合物。此外，L-茶氨酸施用（4 mg/kg，p.o.）持续14天显著增加小鼠纹状体中的谷胱甘肽水平。结果表明，l-茶氨酸提供神经保护，防止氧化应激诱导的神经元损伤的体液分子从星形胶质细胞释放，可能包括谷胱甘肽。
l-Theanine (γ-glutamylethylamide), a component of green tea, is considered to have regulatory and neuroprotective roles in the brain. The present study was designed to determine the effect of l-theanine on excess dopamine-induced neurotoxicity in both cell culture and animal experiments. The primary cultured mesencephalic neurons or co-cultures of mesencephalic neurons and striatal astrocytes were pretreated with l-theanine for 72 h, and then treated with excess dopamine for further 24 h. The cell viability of dopamine neurons and levels of glutathione were evaluated. Excess dopamine-induced neurotoxicity was significantly attenuated by 72 h preincubation with l-theanine in neuron-astrocyte co-cultures but not in neuron-rich cultures. Exposure to l-theanine increased the levels of glutathione in both astrocytes and glial conditioned medium. The glial conditioned medium from l-theanine-pretreated striatal astrocytes attenuated dopamine-induced neurotoxicity and quinoprotein formation in mesencephalic neurons. In addition, replacement of l-glutamate with l-theanine in an in vitro cell-free glutathione-synthesis system produced glutathione-like thiol compounds. Furthermore, l-theanine administration (4 mg/kg, p.o.) for 14 days significantly increased glutathione levels in the striatum of mice. The results suggest that l-theanine provides neuroprotection against oxidative stress-induced neuronal damage by humoral molecules released from astrocytes, probably including glutathione.
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影响因子：
4.8
作者：
Miyazaki, I;Asanuma, M;Ogawa, N
通讯作者：
Ogawa, N
影响因子：
2.9
作者：
Maher, Pamela;Salgado, Karmen F.;Lapchak, Paul A.
通讯作者：
Lapchak, Paul A.
影响因子：
4.7
作者：
Miyazaki, Ikuko;Murakami, Shinki;Asanuma, Masato
通讯作者：
Asanuma, Masato
影响因子：
1.2
作者：
Kurihara, Shigekazu;Shibahara, Susumu;Akiyama, Yukio
通讯作者：
Akiyama, Yukio
影响因子：
6.2
作者：
Miyazaki, Ikuko;Asanuma, Masato;Kita, Taizo
通讯作者：
Kita, Taizo