Group B Streptococcus cpsE Is Required for Serotype V Capsule Production and Aids in Biofilm Formation and Ascending Infection of the Reproductive Tract during Pregnancy.
Group B Streptococcus cpsE Is Required for Serotype V Capsule Production and Aids in Biofilm Formation and Ascending Infection of the Reproductive Tract during Pregnancy.
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DOI：
10.1021/acsinfecdis.1c00182
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发表时间：
2021-09-10
影响因子：
5.3
通讯作者：
Gaddy JA
中科院分区：
文献类型：
--
作者：
Noble K;Lu J;Guevara MA;Doster RS;Chambers SA;Rogers LM;Moore RE;Spicer SK;Eastman AJ;Francis JD;Manning SD;Rajagopal L;Aronoff DM;Townsend SD;Gaddy JA
B族链球菌（GBS）是一种包裹的革兰氏阳性病原体，可导致妊娠期间生殖道的上行感染。这种生物体的荚膜是一种重要的毒力因子，参与多种细胞过程以促进发病。主要由碳水化合物组成，GBS胶囊，其合成由胶囊多糖合成（cps）操纵子驱动。该操纵子内的cpsE基因编码推定的糖基转移酶，其负责将Glc-1-P从UDP-Glc转移至十一异戊二烯基脂质分子。我们推测cpsE基因产物对妊娠期GBS毒力和上行感染很重要。我们的工作表明，GBS cpsE突变体分泌更少的碳水化合物，具有减少的胶囊，并形成更少的生物膜比野生型亲本菌株。我们发现，与亲本菌株相比，ΔcpsE缺失突变体更容易被人胎盘巨噬细胞吸收，并且在小鼠生殖道中侵入和增殖的能力显著减弱。综上所述，这些结果表明，cpsE基因产物是一个重要的毒力因子，有助于GBS定植和侵入妊娠生殖道。
Group B Streptococcus (GBS) is an encapsulated Gram-positive pathogen that causes ascending infections of the reproductive tract during pregnancy. The capsule of this organism is a critical virulence factor that has been implicated in a variety of cellular processes to promote pathogenesis. Primarily comprised of carbohydrates, the GBS capsule, and its synthesis is driven by the capsule polysaccharide synthesis (cps) operon. The cpsE gene within this operon encodes a putative glycosyltransferase that is responsible for the transfer of a Glc-1-P from UDP-Glc to an undecaprenyl lipid molecule. We hypothesized that the cpsE gene product is important for GBS virulence and ascending infection during pregnancy. Our work demonstrates that a GBS cpsE mutant secretes less carbohydrates, has reduced capsule, and forms less biofilm than the wild-type parental strain. We show that compared to the parental strain, the ΔcpsE deletion mutant is more readily taken up by human placental macrophages and has significantly attenuated ability to invade and proliferate in the mouse reproductive tract. Taken together, these results demonstrate that the cpsE gene product is an important virulence factor that aids in GBS colonization and invasion of the gravid reproductive tract.
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影响因子：
2.7
作者：
Kapatai G;Sheppard CL;Al-Shahib A;Litt DJ;Underwood AP;Harrison TG;Fry NK
通讯作者：
Fry NK
影响因子：
4.2
作者：
Parker RE;Laut C;Gaddy JA;Zadoks RN;Davies HD;Manning SD
通讯作者：
Manning SD
影响因子：
4.4
作者：
D'Urzo, Nunzia;Martinelli, Manuele;Maione, Domenico
通讯作者：
Maione, Domenico
影响因子：
16.6
作者：
Kietzman CC;Gao G;Mann B;Myers L;Tuomanen EI
通讯作者：
Tuomanen EI
影响因子：
6.4
作者：
Doster RS;Sutton JA;Rogers LM;Aronoff DM;Gaddy JA
通讯作者：
Gaddy JA