New insights into the genetic networks affecting seed fatty acid concentrations in Brassica napus.
New insights into the genetic networks affecting seed fatty acid concentrations in Brassica napus.
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DOI：
10.1186/s12870-015-0475-8
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发表时间：
2015-03-27
影响因子：
5.3
通讯作者：
Li M
中科院分区：
文献类型：
--
作者：
Wang X;Long Y;Yin Y;Zhang C;Gan L;Liu L;Yu L;Meng J;Li M
油菜(B.napus，AAcc，2n = 38)是世界上最重要的油料作物之一，也是生产生物柴油最常用的油料之一。它的油是多种脂肪酸的混合物，剖析脂肪酸生物合成的遗传网络对提高种子质量具有重要意义。以202个甘蓝型油菜加倍单倍体群体为材料，通过QTL分析研究了甘蓝型油菜脂肪酸生物合成的遗传基础。共检测到72个QTL和大量与10种不同脂肪酸含量相关的上位性互作。在47个QTL的可信区间内，共发现234个与脂肪酸代谢相关的同源基因。其中，分别检测到47个和15个与油菜和甘蓝同源的基因。通过QTL定位、上位性分析和候选基因互作分析，构建了一个控制甘蓝型油菜脂肪酸生物合成的潜在调控途径，包括50个酶编码基因和5个调控因子(LEC1、LEC2、FUS3、WRI1和ABI3)。随后，分析了这五种调控因子与脂肪酸代谢相关基因之间的相互作用。在本研究中，通过QTL分析和电子定位分析，构建了一个潜在的控制脂肪酸的调控途径。这些结果丰富了我们对甘蓝型油菜脂肪酸代谢QTL的认识，为甘蓝型油菜脂肪酸组成的基因工程提供了新的线索。本文的在线版本(doi：10.1186/s12870-0150475-8)包含补充材料，授权用户可以使用。
Rapeseed (B. napus, AACC, 2n = 38) is one of the most important oil seed crops in the world, it is also one of the most common oil for production of biodiesel. Its oil is a mixture of various fatty acids and dissection of the genetic network for fatty acids biosynthesis is of great importance for improving seed quality. The genetic basis of fatty acid biosynthesis in B. napus was investigated via quantitative trail locus (QTL) analysis using a doubled haploid (DH) population with 202 lines. A total of 72 individual QTLs and a large number pairs of epistatic interactions associated with the content of 10 different fatty acids were detected. A total of 234 homologous genes of Arabidopsis thaliana that are involved in fatty acid metabolism were found within the confidence intervals (CIs) of 47 QTLs. Among them, 47 and 15 genes homologous to those of B. rapa and B. oleracea were detected, respectively. After the QTL mapping, the epistatic and the candidate gene interaction analysis, a potential regulatory pathway controlling fatty acid biosynthesis in B. napus was constructed, including 50 enzymes encoded genes and five regulatory factors (LEC1, LEC2, FUS3, WRI1 and ABI3). Subsequently, the interaction between these five regulatory factors and the genes involved in fatty acid metabolism were analyzed. In this study, a potential regulatory pathway controlling the fatty acid was constructed by QTL analysis and in silico mapping analysis. These results enriched our knowledge of QTLs for fatty acids metabolism and provided a new clue for genetic engineering fatty acids composition in B. napus. The online version of this article (doi:10.1186/s12870-015-0475-8) contains supplementary material, which is available to authorized users.
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影响因子：
5.1
作者：
Kachroo, Aardra;Shanklin, John;Kachroo, Pradeep
通讯作者：
Kachroo, Pradeep
影响因子：
7.4
作者：
Beisson, F;Koo, AJK;Ohlrogge, JB
通讯作者：
Ohlrogge, JB
影响因子：
7.4
作者：
Barker, Guy C.;Larson, Tony R.;King, Graham J.
通讯作者：
King, Graham J.
影响因子：
3.1
作者：
Babula, D;Kaczmarek, M;Sadowski, J
通讯作者：
Sadowski, J
影响因子：
5.8
作者：
Arcade, A;Labourdette, A;Joets, J
通讯作者：
Joets, J