Large Sequence-Defined Supramolecules Obtained by the DNA-Guided Assembly of Biohybrid Poly(phosphodiester)s
Large Sequence-Defined Supramolecules Obtained by the DNA-Guided Assembly of Biohybrid Poly(phosphodiester)s
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通过 DNA 引导的生物杂化聚磷酸二酯组装获得大序列定义的超分子
DOI：
10.1021/acs.macromol.0c02581
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发表时间：
2021
期刊：
影响因子：
5.5
通讯作者：
Lutz, Jean-François
中科院分区：
文献类型：
--
作者：
Mondal, Tathagata;Nerantzaki, Maria;Flesch, Kevin;Loth, Capucine;Maaloum, Mounir;Cong, Yidan;Sheiko, Sergei S.;Lutz, Jean-François
研究了生物杂化序列聚磷酸二酯的dna引导组装。这些聚合物含有与单链DNA序列共价连接的长非天然片段。这些生物杂化结构是用核苷和非生物磷酸酯单体自动合成的。利用互补的DNA链，将前体在水缓冲液中组装成线性或星形的上层结构。例如，制备了包含442（352个由3个15个碱基对的双链DNA桥连接的非天然单体）和990（720个由9个15个碱基对的双链DNA桥连接的非天然单体）的线性超分子。还获得了包含488个单体（352个非天然单体通过68个碱基对的四臂连接）的四臂星形结构。形成的超分子通过电泳、紫外光谱和原子力显微镜进行了表征。所有这些技术都证明了预期的超分子的形成，尽管也证明了一些缺陷。这些结果为设计基于信息聚磷酸二酯的二维（2D）和三维（3D）结构开辟了有趣的途径。
The DNA-guided assembly of biohybrid sequence-defined poly(phosphodiester)s was investigated. These polymers contain long non-natural segments covalently connected to single-stranded DNA sequences. These biohybrid structures were synthesized by automated phosphoramidite chemistry using both nucleoside and abiological phosphoramidite monomers. Using complementary DNA strands, the precursors were then assembled in aqueous buffer into linear or star-like superstructures. For instance, linear supramolecules containing 442 (352 non-natural monomers connected by three double-stranded DNA bridges of 15 base pairs) and 990 monomers (720 non-natural monomers connected by nine double-stranded DNA bridges of 15 base pairs) were prepared. A four-arm star structure containing 488 monomers (352 non-natural monomers connected by a four-arm junction of 68 base pairs) was also achieved. The formed supramolecules were characterized by electrophoresis, UV spectroscopy, and atomic force microscopy. All these techniques evidenced the formation of the expected supramolecules, although some defects were also evidenced. These results open up interesting avenues for the design of two-dimensional (2D) and three-dimensional (3D) constructs based on informational poly(phosphodiester)s.
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影响因子：
56.9
作者：
CARUTHERS, MH
通讯作者：
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DOI：
--
发表时间：
1985
期刊：
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作者：
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作者：
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DOI：
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发表时间：
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