Handling method alters the hedonic value of reward in laboratory mice.
Handling method alters the hedonic value of reward in laboratory mice.
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DOI：
10.1038/s41598-018-20716-3
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发表时间：
2018-02-05
影响因子：
4.6
通讯作者：
Rowe C
中科院分区：
文献类型：
--
作者：
Clarkson JM;Dwyer DM;Flecknell PA;Leach MC;Rowe C
小鼠是药物发现和科学研究中应用最广泛的模式动物。因此，必须完善实验室程序和做法，以确保高标准的福利和科学数据质量。最近的研究发现，通过尾巴处理实验室小鼠的标准做法会增加焦虑的行为，这可以通过使用隧道处理小鼠来克服。然而，尽管对小鼠的行为有明显的负面影响，但隧道处理尚未广泛实施。在这项研究中，我们提供了第一个证据，即尾巴处理也减少了小鼠对奖励的反应。快感缺乏是临床抑郁症的核心症状，通过评估啮齿动物如何消耗蔗糖溶液来测量：抑郁的小鼠消耗较少的蔗糖，并且它们在饮酒时的舔击大小（它们的“舔簇大小”）也往往较小。我们发现，与隧道处理的小鼠相比，尾处理的小鼠在两种测量中都显示出更多的快感缺失反应，这表明对奖励的反应性降低，并且可能是更抑郁的状态。我们的发现对实验室小鼠的福利以及科学研究的设计和解释具有重要意义，特别是那些调查或涉及奖励的研究。
Mice are the most widely used model species for drug discovery and scientific research. Consequently, it is important to refine laboratory procedures and practices to ensure high standards of welfare and scientific data quality. Recent studies have identified that the standard practice of handling laboratory mice by their tails increases behaviours indicative of anxiety, which can be overcome by handling mice using a tunnel. However, despite clear negative effects on mice’s behaviour, tunnel handling has yet to be widely implemented. In this study, we provide the first evidence that tail handling also reduces mice’s responses to reward. Anhedonia is a core symptom of clinical depression, and is measured in rodents by assessing how they consume a sucrose solution: depressed mice consume less sucrose and the size of their licking bouts when drinking (their ‘lick cluster sizes’) also tend to be smaller. We found that tail handled mice showed more anhedonic responses in both measures compared to tunnel handled mice, indicative of a decreased responsiveness to reward and potentially a more depressive-like state. Our findings have significant implications for the welfare of laboratory mice as well as the design and interpretation of scientific studies, particularly those investigating or involving reward.
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