High electric field strength two-dimensional peptide separations using a microfluidic device.
High electric field strength two-dimensional peptide separations using a microfluidic device.
复制标题
使用微流体装置的高电场强度二维肽分离。
DOI：
10.1002/elps.201200069
复制
发表时间：
2012-09
期刊：
影响因子：
2.9
通讯作者：
Ramsey, J. Michael
中科院分区：
文献类型：
--
作者：
Henley, W. Hampton;Ramsey, J. Michael
关键词：
已经开发了新的仪器，以提高分辨率，效率和速度的微流控二维分离使用胶束电动色谱（MEKC）耦合到高场强毛细管电泳（CE）。我们小组以前发表的二维分离仪器仅限于8.4 kV的最大电位差，导致第一维的电场强度仅为~200 V/cm。本报告中描述的电路旨在将较高电压电源与快速切换的较低电压电源耦合，以利用两者的最佳特性。施加超过20 kV的电压导致在两个维度上的高电场强度分离，提高分离分辨率、效率和峰值容量，同时减少所需的分析时间。对于模型蛋白质胰蛋白酶消化分离，观察到高达每秒6个肽的检测速率（基于总分析时间）。此外，与具有较长长度通道的微流体芯片结合使用的较高的施加电压保持较高的电场强度，并且对于一些分离产生超过4，000的峰值容量。在这些较长的通道设备中的总分离时间与在低场强下在短通道中获得的总分离时间相当;然而，分辨能力提高了约3倍。
New instrumentation has been developed to improve the resolution, efficiency, and speed of microfluidic two-dimensional separations using micellar electrokinetic chromatography (MEKC) coupled to high field strength capillary electrophoresis (CE). Previously published two-dimensional separation instrumentation from our group was limited to a maximum potential difference of 8.4 kV, resulting in an electric field strength of only ~200 V/cm in the first dimension. The circuit described in this report has been designed to couple a higher voltage supply with a rapidly switching, lower voltage supply to utilize the best features of each. Voltages applied in excess of 20 kV lead to high electric field strength separations in both dimensions, increasing the separation resolution, efficiency, and peak capacity while reducing the required analysis time. Detection rates as high as 6 peptides per second (based on total analysis time) were observed for a model protein tryptic digest separation. Additionally, higher applied voltages used in conjunction with microfluidic chips with longer length channels maintained higher electric field strengths and produced peak capacities of over 4,000 for some separations. Total separation time in these longer channel devices was comparable to that obtained in short channels at low field strength; however, resolving power improved approximately 3 fold.
登录
查看更多内容
影响因子：
7.4
作者：
Ferguson, PD;Goodall, DM;Loran, JS
通讯作者：
Loran, JS
影响因子：
7.4
作者：
FOLEY, JP
通讯作者：
FOLEY, JP
影响因子：
7.4
作者：
Li, Y;Buch, JS;Lee, CS
通讯作者：
Lee, CS
影响因子：
7.4
作者：
Griffiths, SK;Nilson, RH
通讯作者：
Nilson, RH
影响因子：
7.4
作者：
MOORE, AW;JORGENSON, JW
通讯作者：
JORGENSON, JW