Characterization of isolates that cause black rot of crucifers in East Africa
Characterization of isolates that cause black rot of crucifers in East Africa
复制标题
东非十字花科植物黑腐病菌株的鉴定
DOI:
10.1007/s10658-011-9916-x
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发表时间:
2011
影响因子:
1.8
通讯作者:
Mulema J
中科院分区:
文献类型:
--
作者:
Mulema J
A study was conducted in the East African countries of Kenya, Tanzania and Uganda in the months of July and August 2009 with the objectives of assessing the status of black rot and race structure ofXanthomonas campestrispv. campestrisin the three countries. Samples infected with black rot were collected from farmers’ fields mainly fromBrassica oleraceacrops (broccoli, cabbage, cauliflower and kales). A total of 399 farms were surveyed of which 260 were from Kenya, 91 from Tanzania and 48 from Uganda. Following successful isolations, a total of 249 isolates of the causal agent,Xanthomonas campestrispv. campestriswere recovered. Pathogenicity of all isolates was confirmed onB. oleraceasusceptible cultivars Copenhagen Market F1 and Wirosa F1. Sixty of the 250 isolates were race-typed using a differential setBrassicaspp. Only two races, 1 (Kenya and Tanzania) and 4 (Kenya, Tanzania and Uganda) were observed however, another race (5) was observed from one isolate recovered from aB. rapasample obtained from Tanzania in 2003. Genomic fingerprinting with repetitive-PCR revealed clusters that did not depict significant correlations between isolates and geographical location, isolates and host adaptation or isolates and race. However, it did demonstrate existence of genetic differences within the East AfricanX. campestrispv.campestrispopulation indicating that it is not a similar clonal population of the same genetic background.
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DOI:
--
发表时间:
2007
期刊:
影响因子:
--
作者:
H. Wambani;E. M. Nyambati;M. Kamidi;J. Mulati;K. Z. Ahmed
通讯作者:
K. Z. Ahmed
影响因子:
1.8
作者:
J. Vicente;S. J. Roberts
通讯作者:
S. J. Roberts
影响因子:
1.8
作者:
Massomo, SMS;Nielsen, H;Mortensen, CN
通讯作者:
Mortensen, CN
影响因子:
10.7
作者:
Tamura, Koichiro;Dudley, Joel;Kumar, Sudhir
通讯作者:
Kumar, Sudhir
影响因子:
3.2
作者:
J. Vicente;B. Everett;S. J. Roberts
通讯作者:
S. J. Roberts