Characterization of isolates that cause black rot of crucifers in East Africa

Characterization of isolates that cause black rot of crucifers in East Africa
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东非十字花科植物黑腐病菌株的鉴定

DOI:
10.1007/s10658-011-9916-x
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发表时间:
2011
影响因子:
1.8
通讯作者:
Mulema J
Mulema J
中科院分区:
农林科学3区
文献类型:
--
作者:
Mulema J

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2009 年 7 月和 8 月在东非国家肯尼亚、坦桑尼亚和乌干达进行了一项研究,目的是评估黑腐病状况和野油菜黄单胞菌的种族结构。这三个国家的campestris。感染黑腐病的样品主要从甘蓝作物(西兰花、卷心菜、花椰菜和羽衣甘蓝)的农田中采集。总共调查了 399 个农场,其中 260 个来自肯尼亚,91 个来自坦桑尼亚,48 个来自乌干达。成功分离后,总共分离出 249 株致病菌黄单胞菌。油菜被回收。所有分离株的致病性均在 B 上得到证实。甘蓝为感病品种 Copenhagen Market F1 和 Wirosa F1。使用差异组Brassicaspp 对 250 个分离株中的 60 个进行了种族分型。仅观察到两个种族,1(肯尼亚和坦桑尼亚)和 4(肯尼亚、坦桑尼亚和乌干达),然而,从 aB 中回收的一个分离株中观察到另一个种族 (5)。 2003 年从坦桑尼亚获得的 rapa 样本。通过重复 PCR 进行的基因组指纹分析显示,这些簇并未描述分离株与地理位置、分离株与宿主适应或分离株与种族之间的显着相关性。然而,它确实证明了东非X人之间存在遗传差异。 Campestrispv.campestrispopulation 表明它不是具有相同遗传背景的相似克隆群体。
A study was conducted in the East African countries of Kenya, Tanzania and Uganda in the months of July and August 2009 with the objectives of assessing the status of black rot and race structure ofXanthomonas campestrispv. campestrisin the three countries. Samples infected with black rot were collected from farmers’ fields mainly fromBrassica oleraceacrops (broccoli, cabbage, cauliflower and kales). A total of 399 farms were surveyed of which 260 were from Kenya, 91 from Tanzania and 48 from Uganda. Following successful isolations, a total of 249 isolates of the causal agent,Xanthomonas campestrispv. campestriswere recovered. Pathogenicity of all isolates was confirmed onB. oleraceasusceptible cultivars Copenhagen Market F1 and Wirosa F1. Sixty of the 250 isolates were race-typed using a differential setBrassicaspp. Only two races, 1 (Kenya and Tanzania) and 4 (Kenya, Tanzania and Uganda) were observed however, another race (5) was observed from one isolate recovered from aB. rapasample obtained from Tanzania in 2003. Genomic fingerprinting with repetitive-PCR revealed clusters that did not depict significant correlations between isolates and geographical location, isolates and host adaptation or isolates and race. However, it did demonstrate existence of genetic differences within the East AfricanX. campestrispv.campestrispopulation indicating that it is not a similar clonal population of the same genetic background.
对作为肯尼亚西北部小农粮食和收入来源的卷心菜品种进行参与性评估。
DOI: --
发表时间: 2007
期刊:
影响因子: --
作者:
H. Wambani;E. M. Nyambati;M. Kamidi;J. Mulati;K. Z. Ahmed
通讯作者: K. Z. Ahmed
使用rep-PCR 鉴别甜樱桃和野樱桃中的丁香假单胞菌分离株
DOI: --
发表时间: 2007
影响因子: 1.8
作者:
J. Vicente;S. J. Roberts
通讯作者: S. J. Roberts
DOI: 10.1023/a:1026194402701
发表时间: 2003-10-01
影响因子: 1.8
作者:
Massomo, SMS;Nielsen, H;Mortensen, CN
通讯作者: Mortensen, CN
DOI: 10.1093/molbev/msm092
发表时间: 2007-08-01
影响因子: 10.7
作者:
Tamura, Koichiro;Dudley, Joel;Kumar, Sudhir
通讯作者: Kumar, Sudhir
DOI: 10.1094/phyto-96-0735
发表时间: 2006
期刊: Phytopathology
影响因子: 3.2
作者:
J. Vicente;B. Everett;S. J. Roberts
通讯作者: S. J. Roberts