Degradation of elastin in experimental elastase-induced emphysema measured by a radioimmunoassay for desmosine.
Degradation of elastin in experimental elastase-induced emphysema measured by a radioimmunoassay for desmosine.
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通过锁链素放射免疫测定法测量实验性弹性蛋白酶诱导的肺气肿中弹性蛋白的降解。
DOI：
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发表时间：
1983
影响因子：
1.7
通讯作者：
B. Starcher
中科院分区：
文献类型：
--
作者：
C. Kuhn;W. Engleman;M. Chraplyvy;B. Starcher
实验性肺气肿是由一次气管内注射弹性蛋白酶在仓鼠身上产生的，几个月后病情恶化。为了研究这种进展是否是由于持续的弹力溶解，我们用放射免疫分析法(RIA)测量了体内弹性蛋白分解代谢的尿液桥粒排泄量。正常仓鼠排出1.6微克桥粒，相当于每天大约0.4毫克的弹性蛋白周转。在注射25个单位的弹性酶后的前24小时内，桥粒的排泄量增加了三倍，并在几天内迅速恢复正常。6天后桥桥素排泄正常。将注射弹性蛋白酶的金黄地鼠肺组织匀浆在体外孵育，然后用放射免疫分析法检测组织中弹性蛋白酶的活性。该方法足够灵敏，可以检测到0.1微克的弹性蛋白结合酶。弹性酶注射后每隔一段时间取出肺组织，体外溶解桥粒蛋白的量在注射后1小时是对照组的10倍，48小时略有升高，但在7天时与对照组水平相当。这些结果表明，弹性酶诱导的肺气肿的晚期进展并不伴随着弹性溶解的增加。
Experimental emphysema, produced by a single intratracheal injection of elastase in hamsters, progresses in severity over months. To investigate whether this progression is due to continuous elastolysis, we measured the urinary excretion of desmosine by radioimmunoassay (RIA) as a measure of elastin catabolism in vivo. Normal hamster excreted 1.6 microgram of desmosine, equivalent to a daily turnover of approximately 0.4 mg of elastin. During the first 24 hr after injection of 25 units of elastase, excretion of desmosine was increased threefold, rapidly returning to normal over several days. Desmosine excretion was normal after 6 days. Homogenates of lungs from elastase-injected hamsters were incubated in vitro, and the release of soluble desmosine was followed by RIA as a measure of the active elastase in the tissue. The method was sufficiently sensitive to detect 0.1 microgram of enzyme bound to elastin. Desmosine solubilized in vitro from lungs removed at intervals after elastase injection was 10-fold that of control at 1 hr and slightly elevated at 48 hr, but equaled control levels at 7 days. These results indicate that the late progression of elastase-induced emphysema is not accompanied by increased elastolysis.
氯甲基酮对仓鼠弹性蛋白酶诱导的肺气肿进展的影响。
DOI：
10.1164/arrd.1982.126.1.174
发表时间：
1982
期刊：
The American review of respiratory disease
影响因子：
--
作者：
Lucey,EC;Stone,PJ
通讯作者：
Stone,PJ
α-巨球蛋白-弹性蛋白酶复合物在弹性蛋白酶诱导的仓鼠肺气肿发病机制中的作用。
DOI：
10.1172/jci110531
发表时间：
1982
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Stone,PJ;Calore,JD;Snider,GL;Franzblau,C
通讯作者：
Franzblau,C
原弹性蛋白和弹性蛋白肽对 α2-巨球蛋白-蛋白酶复合物降解的抵抗力。
影响因子：
3.9
作者：
Kueppers,F;Abrams,WR;Weinbaum,G;Rosenbloom,J
通讯作者：
Rosenbloom,J