Real-Time Monitoring of a Protein Biomarker.
Real-Time Monitoring of a Protein Biomarker.
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DOI：
10.1021/acssensors.0c01085
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发表时间：
2020-07-24
期刊：
影响因子：
8.9
通讯作者：
Plaxco KW
中科院分区：
文献类型：
--
作者：
Parolo C;Idili A;Ortega G;Csordas A;Hsu A;Arroyo-Currás N;Yang Q;Ferguson BS;Wang J;Plaxco KW
连续和实时监测蛋白质生物标志物的能力将显著提高医学的精确度。然而，目前的蛋白质检测技术，包括酶联免疫吸附试验和横向流动分析，只能提供时间延迟的单一时间点测量，限制了它们引导快速进化的、危及生命的条件下的快速反应的能力。作为回应，我们提出了一种基于电化学适配子的传感器(EAB)，它支持高频、实时的生物标记物测量。具体地说，我们开发了一种基于适体(EAB)的电化学传感器来对抗中性粒细胞明胶酶相关脂蛋白(NGAL)，NGAL是一种蛋白质，如果出现在尿液中的水平超过阈值，则表明急性肾/肾损伤(AKI)。当部署在尿管内时，产生的无试剂、免清洗传感器支持对数小时内临床相关的NGAL浓度进行实时、高频监测。通过提供一个“早期预警系统”，实时测量诊断相关蛋白质水平的能力，如NGAL，可以从根本上改变我们检测、监测和治疗许多重要疾病的方式。
The ability to monitor protein biomarkers continuously and in real-time would significantly advance the precision of medicine. Current protein-detection techniques, however, including ELISA and lateral flow assays, provide only time-delayed, single-time-point measurements, limiting their ability to guide prompt responses to rapidly evolving, life-threatening conditions. In response, here we present an electrochemical aptamer-based sensor (EAB) that supports high-frequency, real-time biomarker measurements. Specifically, we have developed an electrochemical, aptamer-based (EAB) sensor against Neutrophil Gelatinase-Associated Lipocalin (NGAL), a protein that, if present in urine at levels above a threshold value, is indicative of acute renal/kidney injury (AKI). When deployed inside a urinary catheter, the resulting reagentless, wash-free sensor supports real-time, high-frequency monitoring of clinically relevant NGAL concentrations over the course of hours. By providing an “early warning system”, the ability to measure levels of diagnostically relevant proteins such as NGAL in real-time could fundamentally change how we detect, monitor, and treat many important diseases.
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影响因子：
7.4
作者：
Arroyo-Curras, Netzahualcoyotl;Scida, Karen;Plaxco, Kevin W.
通讯作者：
Plaxco, Kevin W.
影响因子：
8.4
作者：
Idili, Andrea;Arroyo-Curras, Netzahualcoyotl;Plaxco, Kevin W.
通讯作者：
Plaxco, Kevin W.
影响因子：
5.4
作者：
Rigla, Mercedes;Hernando, M. Elena;de Leiva, Alberto
通讯作者：
de Leiva, Alberto
影响因子：
9.5
作者：
Shaver, Alexander;Curtis, Samuel D.;Arroyo-Curras, Netzahualcoyotl
通讯作者：
Arroyo-Curras, Netzahualcoyotl
DOI：
10.3390/molecules23040912
发表时间：
2018-04-15
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Somerson J;Plaxco KW
通讯作者：
Plaxco KW