Prospective on Imaging Mass Spectrometry in Clinical Diagnostics.
Prospective on Imaging Mass Spectrometry in Clinical Diagnostics.
复制标题
成像质谱在临床诊断中的前景。
DOI：
10.1016/j.mcpro.2023.100576
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发表时间：
2023-09
影响因子：
7
通讯作者：
Caprioli, Richard M.
中科院分区：
文献类型：
--
作者：
Moore, Jessica L.;Patterson, Nathan Heath;Norris, Jeremy L.;Caprioli, Richard M.
成像质谱（IMS）是一种用于空间驱动研究的分子技术，提供来自组织切片的分子图谱。本文综述了基质辅助激光解吸电离（MALDI）IMS及其在临床实验室中的应用进展。多年来，MALDI质谱法已用于细菌分类和进行其他基于平板的分析的批量分析。然而，在组织活检中的空间数据的临床应用用于诊断和诊断仍然是分子诊断中的新兴机会。这项工作考虑了空间驱动的质谱方法用于临床诊断，并解决了新的基于成像的测定，包括分析物选择，质量控制/保证指标，数据再现性，数据分类和数据评分方面。有必要实施这些任务，以将IMS严格转化为临床实验室;然而，这需要将IMS引入临床实验室的详细标准化方案，以提供可靠和可重复的结果，为患者护理提供信息和指导。我们审查使用IMS的发展新的分子检测我们讨论的IMS检测的质量控制和质量保证指标我们探索IMS的诊断和预后的临床应用我们讨论的监管要求，必要的实际实施成像质谱是一种新兴的技术，用于临床应用，它提供了空间的分子数据内的组织活检的患者诊断和诊断。本文考虑了使用该技术进行新的基于成像的测定的各个方面，包括分析物选择，质量控制/保证指标，数据再现性，数据分类和数据评分。
Imaging mass spectrometry (IMS) is a molecular technology utilized for spatially driven research, providing molecular maps from tissue sections. This article reviews matrix-assisted laser desorption ionization (MALDI) IMS and its progress as a primary tool in the clinical laboratory. MALDI mass spectrometry has been used to classify bacteria and perform other bulk analyses for plate-based assays for many years. However, the clinical application of spatial data within a tissue biopsy for diagnoses and prognoses is still an emerging opportunity in molecular diagnostics. This work considers spatially driven mass spectrometry approaches for clinical diagnostics and addresses aspects of new imaging-based assays that include analyte selection, quality control/assurance metrics, data reproducibility, data classification, and data scoring. It is necessary to implement these tasks for the rigorous translation of IMS to the clinical laboratory; however, this requires detailed standardized protocols for introducing IMS into the clinical laboratory to deliver reliable and reproducible results that inform and guide patient care. We review the use of IMS for the development of new molecular assays We discuss quality control and quality assurance metrics for IMS assays We explore IMS for diagnostic and prognostic clinical applications We discuss regulatory requirements necessary for practical implementation Imaging mass spectrometry is an emerging technology for clinical applications in that it provides spatial molecular data within a tissue biopsy for patient diagnoses and prognoses. This article considers various aspects of the use of this technology for new imaging-based assays that include analyte selection, quality control/assurance metrics, data reproducibility, data classification, and data scoring.
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影响因子：
4.6
作者：
Bankhead P;Loughrey MB;Fernández JA;Dombrowski Y;McArt DG;Dunne PD;McQuaid S;Gray RT;Murray LJ;Coleman HG;James JA;Salto-Tellez M;Hamilton PW
通讯作者：
Hamilton PW
影响因子：
2.5
作者：
Borren, Nienke Z.;Ananthakrishnan, Ashwin N.
通讯作者：
Ananthakrishnan, Ashwin N.
DOI：
10.1097/jto.0b013e31826c1155
发表时间：
2012-11
期刊：
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer
影响因子：
--
作者：
Carbone DP;Ding K;Roder H;Grigorieva J;Roder J;Tsao MS;Seymour L;Shepherd FA
通讯作者：
Shepherd FA
影响因子：
7.4
作者：
Boskamp, Tobias;Lachmund, Delf;Maass, Peter
通讯作者：
Maass, Peter
影响因子：
9.3
作者：
Darebna, Petra;Spicka, Jan;Pompach, Petr
通讯作者：
Pompach, Petr