Bacteria-instructed synthesis of polymers for self-selective microbial binding and labelling.
Bacteria-instructed synthesis of polymers for self-selective microbial binding and labelling.
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DOI：
10.1038/nmat3949
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发表时间：
2014-07
期刊：
影响因子：
41.2
通讯作者：
Alexander, Cameron
中科院分区：
文献类型：
--
作者：
Magennis, E. Peter;Fernandez-Trillo, Francisco;Sui, Cheng;Spain, Sebastian G.;Bradshaw, David J.;Churchley, David;Mantovani, Giuseppe;Winzer, Klaus;Alexander, Cameron
病原性细菌菌株的检测和灭活仍然是重要的治疗目标。因此，对于诊断或抗感染应用，需要能够选择性地结合特定微生物的材料，但是其可以由简单且廉价的结构单元形成。在这里，我们利用细菌氧化还原系统来诱导细胞表面合成单体的铜介导的自由基聚合，原位产生与产生它们的微生物强烈结合的聚合物。这种“细菌指导的合成”可以用多种微生物菌株进行，并且我们表明产生的聚合物是“指导”细胞类型的自选择性结合剂。我们进一步扩展了细菌氧化还原化学，将荧光报告分子直接“点击”到一系列临床分离菌株表面的聚合物上，从而实现快速、简便、同时的病原体结合和可视化。
The detection and inactivation of pathogenic strains of bacteria continues to be an important therapeutic goal. Hence, there is a need for materials that can bind selectively to specific microorganisms, for diagnostic or anti-infective applications, but which can be formed from simple and inexpensive building blocks. Here, we exploit bacterial redox systems to induce a copper-mediated radical polymerisation of synthetic monomers at cell surfaces, generating polymers in situ that bind strongly to the microorganisms which produced them. This ‘bacteria-instructed synthesis’ can be carried out with a variety of microbial strains, and we show that the polymers produced are self-selective binding agents for the ‘instructing’ cell types. We further expand on the bacterial redox chemistries to ‘click’ fluorescent reporters onto polymers directly at the surfaces of a range of clinical isolate strains, allowing rapid, facile and simultaneous binding and visualisation of pathogens.
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影响因子：
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作者：
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通讯作者：
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通讯作者：
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作者：
Aherne, A;Alexander, C;Vulfson, EN
通讯作者：
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