Favorable allele mining and breeding utilization of ALK in rice
Favorable allele mining and breeding utilization of ALK in rice
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水稻中 ALK 的有利等位基因挖掘和育种利用
DOI：
10.1007/s11032-020-01183-z
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发表时间：
2020-11
影响因子：
3.1
通讯作者：
Wang Yuping
中科院分区：
文献类型：
--
作者：
Zheng Ling;Liu Pin;Zhang Shangxing;Li Jialian;Muhammad Yaseen;Yun Yifan;Hu Li;Xue Fengying;Wang Yangkai;Yuan Hua;Chen Weilan;Qin Peng;Ma Bingtian;Li Shigui;Tu Bin;Wang Yuping
ALK基因是控制水稻糊化温度的关键基因，至少有3个ALK等位基因决定了水稻品种间糊化温度的多样性。但启动子区的序列多态性是否也与GT相关尚不清楚。在本研究中，我们分析了ALK启动子区域的序列变异，主要鉴定了两种类型的启动子，ALK I型启动子在成千上万的籼稻和粳稻品种中占优势，而ALK II型启动子在Aus/boro和Basmati生态型品种中占优势。通过对不同ALK等位基因的CSSLs进行表型分析、品质鉴定和启动子活性测定，结果表明Ⅱ型启动子下调ALK的表达水平，从而影响ASV值; ALKQ 1882具有Ⅱ型启动子区和ALK基因4329/4330 bp的TT SNP位点，是一个在稻米品质改良中具有优势的ALK等位基因。杂交三倍体胚乳转录检测表明，ALK在双亲基因拷贝上同时表达，可以通过双亲基因型估计杂交水稻品种的ASV。更重要的是，本研究开发了一个新的dCAPS标记，用于区分TT基因4329/4330 bp的功能SNP，该标记可以在低检测成本的情况下，以高精度检测小麦品质。
ALKis the key gene of controlling rice gelatinization temperature (GT), and at least threeALKalleles are thought to be responsible for the diversity of GT among rice cultivars. But whether the sequence polymorphism of the promoter region also associated with GT is not clearly studied. In this study, we analyzed sequence variation in the promoter region ofALKand mainly two types of promoter were identified.ALKtype I promoter prevails among thousands ofindicaandjaponicarice varieties, whileALKtype II promoter predominates over varieties from Aus/boro and Basmati ecotypes. Phenotypic analysis and quality characterization of CSSLs with different alleles ofALKand promoter activity assays revealed that type II promoter downregulates the expression level ofALKand subsequently, negatively affects ASV values.ALKQ1882, owning type II promoter region and TT SNP at 4329/4330 bp ofALKgene, was identified as a rare and eliteALKallele for rice quality improvement. Transcriptional detection in hybrid triploid endosperm revealed thatALKis simultaneously expressed by gene copes from both parents, the ASV of hybrid rice varieties can be estimated through the genotypes of two parents. More importantly, a new dCAPS marker was developed to discriminate the functional SNP of TT at 4329/4330 bp, which is an efficient tool for improving the grain quality with high accuracy on low testing cost.
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影响因子：
5.4
作者：
Bao, J. S.;Corke, H.;Sun, M.
通讯作者：
Sun, M.
可溶性淀粉合酶 II 基因家族的等位基因变异导致水稻 (Oryza sativa L.) 谷物品质和淀粉特性的变化
影响因子：
2
作者：
Fan, X. Y.;Guo, M.;Yan, C. J.
通讯作者：
Yan, C. J.
影响因子：
9.4
作者：
Yu, Guoqin;Olsen, Kenneth M.;Schaal, Barbara A.
通讯作者：
Schaal, Barbara A.
影响因子：
11.4
作者：
Gao, Zhenyu;Zeng, Dali;Qian, Qian
通讯作者：
Qian, Qian
DOI：
10.1071/fp04009
发表时间：
2004-08
期刊：
Functional plant biology : FPB
影响因子：
--
作者：
T. Umemoto;N. Aoki;Hong-Xuan Lin;Yasunori Nakamura;N. Inouchi;Youichiro Sato;M. Yano;H. Hirabayashi;S. Maruyama
通讯作者：
T. Umemoto;N. Aoki;Hong-Xuan Lin;Yasunori Nakamura;N. Inouchi;Youichiro Sato;M. Yano;H. Hirabayashi;S. Maruyama