SoxAX Binding Protein, a Novel Component of the Thiosulfate-Oxidizing Multienzyme System in the Green Sulfur Bacterium Chlorobium tepidum
SoxAX Binding Protein, a Novel Component of the Thiosulfate-Oxidizing Multienzyme System in the Green Sulfur Bacterium Chlorobium tepidum
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SoxAX 结合蛋白，绿硫细菌温热氯菌中硫代硫酸盐氧化多酶系统的新成分
DOI：
10.1128/jb.00634-08
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发表时间：
2008
影响因子：
3.2
通讯作者：
Kazuhito Inoue
中科院分区：
文献类型：
--
作者：
Takuro Ogawa;Toshinari Furusawa;Ryohei Nomura;D. Seo;Naomi Hosoya;H. Sakurai;Kazuhito Inoue
摘要从光合绿色硫细菌Chlorobium tepidum（pro synon.暗黄绿杆菌），我们已经纯化了三个不可或缺的因素的硫代硫酸盐依赖性还原的小，单血红素细胞色素c554。这些是在各种硫代硫酸盐氧化细菌中发现的硫氧化（Sox）系统因子的同系物。第一个因子是SoxYZ，它作为反应中间体的受体。第二个因素是单体SoxB，其被提议催化硫酸盐从SoxYZ结合的硫代硫酸盐的氧化产物的水解裂解。第三个因子是三聚体细胞色素c551，由单血红素细胞色素SoxA、单血红素细胞色素SoxX和假设的开放阅读框CT 1020的产物组成。后三种组分分别在大肠杆菌细胞中表达并纯化至均一。在其他两个Sox因子的存在下，重组SoxA和SoxX表现出低，但可辨别的硫代硫酸盐依赖性细胞色素c 554还原活性。进一步添加重组CT 1020蛋白大大增加了活性，并且总活性与天然SoxAX-CT 1020蛋白复合物的活性一样高。重组CT 1020蛋白参与与SoxA和SoxX形成紧密复合物，并将其称为SAXB（SoxAX结合蛋白）。同源物的SAXB基因被发现在许多菌株中，包括大约三分之一的硫代硫酸盐氧化细菌的SOX基因簇序列已被存放到目前为止，范围在Chlorobiaciae，Chromatiaceae，Hydrogenophilaceae，Oceanoacellaceae等。每个推导的SoxA和SoxX蛋白的这些细菌构成的组，是不同的那些发现在细菌中，显然缺乏SAXB基因同源物。
ABSTRACT From the photosynthetic green sulfur bacterium Chlorobium tepidum (pro synon. Chlorobaculum tepidum), we have purified three factors indispensable for the thiosulfate-dependent reduction of the small, monoheme cytochrome c 554. These are homologues of sulfur-oxidizing (Sox) system factors found in various thiosulfate-oxidizing bacteria. The first factor is SoxYZ that serves as the acceptor for the reaction intermediates. The second factor is monomeric SoxB that is proposed to catalyze the hydrolytic cleavage of sulfate from the SoxYZ-bound oxidized product of thiosulfate. The third factor is the trimeric cytochrome c 551, composed of the monoheme cytochrome SoxA, the monoheme cytochrome SoxX, and the product of the hypothetical open reading frame CT1020. The last three components were expressed separately in Escherichia coli cells and purified to homogeneity. In the presence of the other two Sox factors, the recombinant SoxA and SoxX showed a low but discernible thiosulfate-dependent cytochrome c 554 reduction activity. The further addition of the recombinant CT1020 protein greatly increased the activity, and the total activity was as high as that of the native SoxAX-CT1020 protein complex. The recombinant CT1020 protein participated in the formation of a tight complex with SoxA and SoxX and will be referred to as SAXB (SoxAX binding protein). Homologues of the SAXB gene are found in many strains, comprising roughly about one-third of the thiosulfate-oxidizing bacteria whose sox gene cluster sequences have been deposited so far and ranging over the Chlorobiaciae, Chromatiaceae, Hydrogenophilaceae, Oceanospirillaceae, etc. Each of the deduced SoxA and SoxX proteins of these bacteria constitute groups that are distinct from those found in bacteria that apparently lack SAXB gene homologues.
影响因子：
2.9
作者：
BERRY, EA;TRUMPOWER, BL
通讯作者：
TRUMPOWER, BL
影响因子：
2.9
作者：
Fabienne Verte;Vesna Kostanjevecki;L. D. Smet;Terrance E. Meyer;M. Cusanovich;J. V. Beeumen
通讯作者：
Fabienne Verte;Vesna Kostanjevecki;L. D. Smet;Terrance E. Meyer;M. Cusanovich;J. V. Beeumen
来自光养绿硫细菌 Chlorobium limicola（Tassajara 菌株）的可溶性细胞色素 c-551 的一级结构揭示了一种新型 c 型细胞色素。
DOI：
10.1021/bi9806706
发表时间：
1998
期刊：
Biochemistry.
影响因子：
--
作者：
Klarskov,K;Verte,F;VanDriessche,G;Meyer,TE;Cusanovich,MA;VanBeeumen,J
通讯作者：
VanBeeumen,J