Detection of femtomolar proteins by nonfluorescent ZnS nanocrystal clusters.
Detection of femtomolar proteins by nonfluorescent ZnS nanocrystal clusters.
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DOI：
10.1021/ac202910y
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发表时间：
2012-02-07
影响因子：
7.4
通讯作者：
Zhong, Wenwan
中科院分区：
文献类型：
--
作者：
Yao, Jingjing;Han, Xiaogang;Zeng, Shang;Zhong, Wenwan
Cation exchange (CX) in the non-fluorescent ZnS nanocrystal clusters (NCCs) was employed to detect trace biomolecules with immunoassays. The NCCs were porous, and allowed fast cation exchange reaction to release an ultra-large number of Zn2+ from each cluster that turned on the Zn-responsive dyes for fluorescence detection. The ZnS NCCs were highly stable in biological buffers and more biocompatible than quantum dots. Zn2+ release efficiency and target binding by NCCs with average diameters of 44 nm, 86 nm, and 144 nm were investigated. The smallest NCCs exhibited the highest CX efficiency because of its larger surface area and bigger pores inside the cluster structure, and 71.0% of the enclosed Zn2+ were freed by CX with 2-minute microwave irradiation. They also experienced the least space hindrance and the fastest rate when binding to target molecules immobilized on surface. When the 44-nm NCCs were used to detect IgE in a sandwich assay, the limit of detection (LOD) was 5 pg/mL (33 fM), 1,000 times better than that of ELISA. Our results well demonstrate that CX in the ZnS NCCs is superior to the conventional signaling strategies in its high amplification efficiency, robustness, and biocompatibility.
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影响因子：
3.3
作者：
Qian, HF;Qiu, X;Ren, JC
通讯作者：
Ren, JC
影响因子：
3.9
作者：
Nguyen, Thanh-Dinh;Do, Trong-On
通讯作者：
Do, Trong-On
DOI：
10.1073/pnas.0601211103
发表时间：
2006-03-28
影响因子：
11.1
作者：
Edgar, R;McKinstry, M;Adhya, S
通讯作者：
Adhya, S
DOI：
10.1080/01971529909349349
发表时间：
1999-01-01
期刊：
JOURNAL OF IMMUNOASSAY
影响因子：
--
作者：
Khatkhatay, MI;Desai, M
通讯作者：
Desai, M
影响因子：
15
作者：
Gee, KR;Zhou, ZL;Kennedy, R
通讯作者：
Kennedy, R