A WD40 Repeat Protein from Camellia sinensis Regulates Anthocyanin and Proanthocyanidin Accumulation through the Formation of MYB⁻bHLH⁻WD40 Ternary Complexes.
A WD40 Repeat Protein from Camellia sinensis Regulates Anthocyanin and Proanthocyanidin Accumulation through the Formation of MYB⁻bHLH⁻WD40 Ternary Complexes.
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茶树中的 WD40 重复蛋白通过形成 MYB-bHLH-WD40 三元复合物调节花青素和原花青素的积累
DOI：
10.3390/ijms19061686
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发表时间：
2018-06-06
影响因子：
5.6
通讯作者：
Xia T
中科院分区：
文献类型：
--
作者：
Liu Y;Hou H;Jiang X;Wang P;Dai X;Chen W;Gao L;Xia T
黄烷-3-醇和低聚原花青素（PA）是绿色茶（Camellia sinensis）中的主要营养多酚类物质，对人体健康具有重要的作用。迄今为止，绿色茶中黄烷-3-醇生物合成的调控机制仍有待研究。在此，我们报告了一个C。CsWD 40与成髓细胞瘤（MYB）和碱性螺旋-环-螺旋（bHLH）转录因子（TF）相互作用，调节黄烷-3-醇的生物合成。从叶片中克隆了CsWD 40的全长cDNA，推测编码342个氨基酸。体外酵母双杂交实验表明，CsWD 40与两个bHLH TF（CsGL 3和CsTT 8）和两个MYB TF（CsAN 2和CsMYB 5e）相互作用。CsWD 40在拟南芥透明种皮1（ttg 1）中的过表达恢复了正常的毛状体和种皮发育。CsWD 40单独在烟草中的异位表达导致转基因花瓣中花青素的显着增加。然后，CsWD 40与CsMYB 5e在烟草植物中共表达，以增加花青素和PA的水平。此外，基因表达分析表明，CsWD 40在茶树中的表达可以诱导多种非生物胁迫。综上所述，这些数据提供了坚实的证据表明，CsWD 40与bHLH和MYB TF合作，形成三元WBM复合物，以调节花青素苷，PA生物合成和毛状体发育。
Flavan-3-ols and oligomeric proanthocyanidins (PAs) are the main nutritional polyphenols in green tea (Camellia sinensis), which provide numerous benefits to human health. To date, the regulatory mechanism of flavan-3-ol biosynthesis in green tea remains open to study. Herein, we report the characterization of a C. sinensis tryptophan-aspartic acid repeat protein (CsWD40) that interacts with myeloblastosis (MYB) and basic helix-loop-helix (bHLH) transcription factors (TFs) to regulate the biosynthesis of flavan-3-ols. Full length CsWD40 cDNA was cloned from leaves and was deduced to encode 342 amino acids. An in vitro yeast two-hybrid assay demonstrated that CsWD40 interacted with two bHLH TFs (CsGL3 and CsTT8) and two MYB TFs (CsAN2 and CsMYB5e). The overexpression of CsWD40 in Arabidopsis thaliana transparent testa glabra 1 (ttg1) restored normal trichome and seed coat development. Ectopic expression of CsWD40 alone in tobacco resulted in a significant increase in the anthocyanins of transgenic petals. CsWD40 was then coexpressed with CsMYB5e in tobacco plants to increase levels of both anthocyanins and PAs. Furthermore, gene expression analysis revealed that CsWD40 expression in tea plants could be induced by several abiotic stresses. Taken together, these data provide solid evidence that CsWD40 partners with bHLH and MYB TFs to form ternary WBM complexes to regulate anthocyanin, PA biosynthesis, and trichome development.
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影响因子：
7.4
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