Expression of the C-type lectins DC-SIGN or L-SIGN alters host cell susceptibility for the avian coronavirus, infectious bronchitis virus.
Expression of the C-type lectins DC-SIGN or L-SIGN alters host cell susceptibility for the avian coronavirus, infectious bronchitis virus.
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DOI：
10.1016/j.vetmic.2012.01.011
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发表时间：
2012-06-15
影响因子：
3.3
通讯作者：
Whittaker GR
中科院分区：
文献类型：
--
作者：
Zhang Y;Buckles E;Whittaker GR
传染性支气管炎病毒（Infectious bronchitis virus，IBV）是一种禽类冠状病毒，给养禽业造成巨大的经济损失。该病毒主要感染呼吸道上皮，但也可以在其他器官中检测到。尚未发现该病毒的功能性受体，IBV的野毒株不感染常规细胞系。最近，已经显示C型凝集素DC-SIGN/L-SIGN可以促进几种冠状病毒的进入。在这里，我们检查DC-SIGN/L-SIGN是否是IBV的进入决定因素。我们表明，通过将人DC-SIGN/L-SIGN引入非允许细胞，IBV的感染显著增加。DC-SIGN介导的感染被甘露聚糖和抗凝集素抗体抑制，并且不依赖于细胞上的唾液酸水平。不同血清型的IBV感染也有不同程度的增强。我们的研究结果表明，即使在缺乏禽类特异性受体的情况下，DC-SIGN样凝集素也能够介导有效的IBV感染。
Infectious bronchitis virus (IBV), an avian coronavirus, is a cause of great economic loss in the poultry industry. The virus mainly infects respiratory epithelium, but can be also detected in other organs. The functional receptor for the virus has not been found and field strains of IBV do not infect conventional cell lines. Recently, it has been shown that the C-type lectins DC-SIGN/L-SIGN can promote entry of several coronaviruses. Here we examine whether DC-SIGN/L-SIGN are entry determinants for IBV. We show that by introducing human DC-SIGN/L-SIGN into non-permissive cells, infection by the IBV is dramatically increased. DC-SIGN mediated infection was inhibited by mannan and anti-lectin antibodies, and was independent of sialic acid levels on the cell. Enhancement of IBV infection also occurred for different serotypes of IBV. Our findings demonstrated that even in the absence of avian-specific receptor, DC-SIGN-like lectins are capable of mediating efficient IBV infection.
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