Requirement of R-SNAREs VAMP721 and VAMP722 for the gametophyte activity, embryogenesis and seedling root development in Arabidopsis
Requirement of R-SNAREs VAMP721 and VAMP722 for the gametophyte activity, embryogenesis and seedling root development in Arabidopsis
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R-SNARE VAMP721 和 VAMP722 对拟南芥配子体活性、胚胎发生和幼苗根发育的要求
DOI：
10.1007/s10725-015-0035-0
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发表时间：
2015-02
影响因子：
4.2
通讯作者：
J. Li
中科院分区：
文献类型：
--
作者：
W. Li;T. Wang;F. Zheng;J. Li
转运囊泡和靶膜之间的膜融合是由 SNARE 复合体介导的，SNARE 复合体是囊泡运输的关键调节因子。功能性 SNARE 复合体由 Q-SNARE 和 R-SNARE 提供的四个盘绕螺旋束组成。在这里，我们分析了拟南芥 R-SNARE VAMP721 和 VAMP722。互交表明，vamp721vamp722等位基因的传播通过VAMP721−/−VAMP722+/-植物的配子体略有减少，并且通过VAMP721+/-VAMP722−/−植物的花粉明显阻断。胚胎发生观察表明，vamp721vamp722突变导致胚胎形态异常，如子叶发育不对称、子叶发育三个、子叶和根展开、胚胎发育部分停滞在球状期等。此外，双突变体幼苗长出的未发育根表现出分生组织区减少、QC细胞紊乱、细胞层模式和细胞排列排列紊乱。共焦图像显示 VAMP721 和 VAMP722 在整个根中表达。综上所述，我们的结果表明 VAMP721 和 VAMP722 参与拟南芥配子体传递、胚胎发育和幼苗根生长。
Membrane fusion between transport vesicles and target membranes is mediated by SNARE complex, a key regulator of vesicular traffic. A functional SNARE complex consists of four coiled-coil helical bundles supplied by Q-SNARE and R-SNARE. Here, we analyze the Arabidopsis R-SNAREs VAMP721 and VAMP722. Reciprocal crosses indicated that the transmission of vamp721vamp722 allele was slightly reduced through gametophytes of VAMP721−/−VAMP722+/− plants and obviously blocked through pollen of VAMP721+/−VAMP722−/− plants. The observation of embryogenesis showed that vamp721vamp722 mutations resulted in abnormal embryo morphology, such as embryos with asymmetric developing cotyledons, three developing cotyledons, unfolded cotyledons and roots, and partial arrested embryo development at globular stage. Moreover, double mutant seedlings grew rudimentary roots displaying reduced meristem zone, disorganized QC cells, and disordered cell layer pattern and cell file alignment. Confocal images revealed that VAMP721 and VAMP722 were expressed throughout whole root. Taken together, our results suggest that VAMP721 and VAMP722 are involved in gametophyte transmission, embryo development and seedling root growth in Arabidopsis.
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影响因子：
64.8
作者：
SOLLNER, T;WHITEHART, SW;ROTHMAN, JE
通讯作者：
ROTHMAN, JE
影响因子：
3.7
作者：
Cazzonelli CI;Vanstraelen M;Simon S;Yin K;Carron-Arthur A;Nisar N;Tarle G;Cuttriss AJ;Searle IR;Benkova E;Mathesius U;Masle J;Friml J;Pogson BJ
通讯作者：
Pogson BJ
影响因子：
11.6
作者：
Abidur Rahman;Maho Takahashi;Kyohei Shibasaki;Shuang Wu;T. Inaba;S. Tsurumi;T. Baskin
通讯作者：
Abidur Rahman;Maho Takahashi;Kyohei Shibasaki;Shuang Wu;T. Inaba;S. Tsurumi;T. Baskin
DOI：
10.1073/pnas.0604421103
发表时间：
2006-11-21
影响因子：
11.1
作者：
Leshem, Yehoram;Melamed-Book, Naomi;Levine, Alex
通讯作者：
Levine, Alex
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF