Characterization of wall-associated kinase/wall-associated kinase-like (WAK/WAKL) family in rose (Rosa chinensis) reveals the role of RcWAK4 in Botrytis resistance.
Characterization of wall-associated kinase/wall-associated kinase-like (WAK/WAKL) family in rose (Rosa chinensis) reveals the role of RcWAK4 in Botrytis resistance.
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玫瑰 (Rosa chinensis) 中壁相关激酶/壁相关激酶样 (WAK/WAKL) 家族的表征揭示了 RcWAK4 在灰霉病抗性中的作用
DOI：
10.1186/s12870-021-03307-9
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发表时间：
2021-11-10
影响因子：
5.3
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Liu X;Wang Z;Tian Y;Zhang S;Li D;Dong W;Zhang C;Zhang Z
壁相关激酶（Wall-associated kinase， WAK）/类壁相关激酶（Wall-associated kinase， WAKL）是受体样激酶（receptor like kinase， RLK）的亚家族之一。虽然已有研究报道了WAK/WAKL在植物细胞伸长、生物和非生物胁迫响应中发挥重要作用，但对玫瑰中RcWAK/RcWAKL的系统研究尚不存在。在本研究中，我们鉴定了玫瑰（Rosa chinensis）基因组中共68个RcWAK/RcWAKL基因家族成员。RcWAKs包含细胞外半乳糖醛酸结合结构域和钙结合表皮生长因子（EGF）样结构域，以及细胞内激酶结构域。rcwakl在其细胞外区域缺少钙结合的egf样结构域或半乳糖醛酸结合结构域。系统发育分析表明，RcWAK/RcWAKL基因家族被划分为5个群体，这些RcWAK/RcWAKL基因不均匀地分布在玫瑰的7条染色体上。其中12个RcWAK/RcWAKL基因被葡萄孢杆菌接种的玫瑰花瓣显著上调，其中RcWAK4表达最强烈。病毒诱导的RcWAK4基因沉默增加了玫瑰花瓣对灰葡萄球菌的敏感性。结果表明，RcWAK4参与了玫瑰花瓣对灰孢杆菌的抗性。本研究为进一步研究RcWAK/RcWAKL基因家族的功能以及玫瑰抗灰孢杆菌的育种研究提供了有益的信息。在线版本包含补充材料，可在10.1186/s12870-021-03307-9获得。
Wall-associated kinase (WAK)/WAK-like (WAKL) is one of the subfamily of receptor like kinases (RLK). Although previous studies reported that WAK/WAKL played an important role in plant cell elongation, response to biotic and abiotic stresses, there are no systematic studies on RcWAK/RcWAKL in rose. In this study, we identified a total of 68 RcWAK/RcWAKL gene family members within rose (Rosa chinensis) genome. The RcWAKs contained the extracellular galacturonan-binding domain and calcium-binding epidermal growth factor (EGF)-like domain, as well as an intracellular kinase domains. The RcWAKLs are missing either calcium-binding EGF-like domain or the galacturonan-binding domain in their extracellular region. The phylogenetic analysis showed the RcWAK/RcWAKL gene family has been divided into five groups, and these RcWAK/RcWAKL genes were unevenly distributed on the 7 chromosomes of rose. 12 of RcWAK/RcWAKL genes were significantly up-regulated by Botrytis cinerea-inoculated rose petals, where RcWAK4 was the most strongly expressed. Virus induced gene silencing of RcWAK4 increased the rose petal sensitivity to B. cinerea. The results indicated RcWAK4 is involved in the resistance of rose petal against B. cinerea. Our study provides useful information to further investigate the function of the RcWAK/RcWAKL gene family and breeding research for resistance to B. cinerea in rose. The online version contains supplementary material available at 10.1186/s12870-021-03307-9.
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影响因子：
5.6
作者：
Underwood W
通讯作者：
Underwood W
影响因子：
5.6
作者：
Newman MA;Sundelin T;Nielsen JT;Erbs G
通讯作者：
Erbs G
棉花WAK基因家族的全基因组表征及植物激素处理下的表达分析
影响因子：
4.4
作者：
Dou L;Li Z;Shen Q;Shi H;Li H;Wang W;Zou C;Shang H;Li H;Xiao G
通讯作者：
Xiao G
DOI：
10.1360/062004-37
发表时间：
2005-10-01
期刊：
SCIENCE IN CHINA SERIES C-LIFE SCIENCES
影响因子：
--
作者：
Ma, N;Cai, L;Gao, JP
通讯作者：
Gao, JP
分离花瓣盘测定和病毒诱导的基因沉默促进了月季花灰霉病抗性的研究
影响因子：
8.7
作者：
Cao, Xiaoqian;Yan, Huijun;Zhang, Zhao
通讯作者：
Zhang, Zhao