Comparative methylomics between domesticated and wild silkworms implies possible epigenetic influences on silkworm domestication.
Comparative methylomics between domesticated and wild silkworms implies possible epigenetic influences on silkworm domestication.
复制标题
家蚕和野生蚕之间的比较甲基组学暗示了家蚕驯化可能的表观遗传影响
DOI：
10.1186/1471-2164-14-646
复制
发表时间：
2013-09-23
期刊：
影响因子：
4.4
通讯作者：
Wang W
中科院分区：
文献类型：
--
作者：
Xiang H;Li X;Dai F;Xu X;Tan A;Chen L;Zhang G;Ding Y;Li Q;Lian J;Willden A;Guo Q;Xia Q;Wang J;Wang W
背景与野生物种不同，野生物种通常在长期的自然选择中进化出表型，而驯化物种通过人工选择在相对较短的时间内迅速获得了人类偏好的农艺性状。在驯化条件下，可以观察到许多性状，这些性状不仅仅是由于环境的改变。在蚕的例子中，除了遗传差异，表观遗传差异是否在驯化中发挥了作用是一个没有答案的问题。家蚕仍然是一个谜，因为它有两个DNA甲基转移酶（DNMT 1和DNMT 2），但它们的功能尚不清楚。即使在特别是广泛分布的DNMT 1的功能仍然是未知的昆虫在general.ResultsBy胚胎RNA干扰，我们揭示了，击倒的蚕Dnmt 1导致孵化率下降，提供了第一个直接的实验证据的功能意义的insectDnmt 1。鉴于这一事实以及DNA甲基化与家蚕基因表达相关以及驯化生物的某些农艺性状不稳定的事实，我们综合比较了3种家蚕（家蚕）和4种野生家蚕（家蚕）的丝腺甲基化组，以确定两者之间的差异甲基化基因。我们观察到2倍多分化的甲基化胞嘧啶（MCs）在驯化的家蚕相比，野生的，这表明在驯化过程中增加DNA甲基化的趋势。进一步对更多的家蚕品种和野生家蚕品种进行研究，缩小了家蚕品种的表型突变范围，并鉴定出一批差异基因。一个这样的基因在驯化动物中表现出去甲基化，相应地显示出较低的基因表达，更有趣的是，经历了选择性清除。甲甲基化增加的基因似乎会导致在驯化和itsDrosophilahomolog的功能是必不可少的细胞体积调节，表明可能与扩大的丝腺在驯化silkworm.ConclusionsOur结果暗示表观遗传的影响，在驯化过程中工作，这给了深入了解长期的历史争议，后天遗传。
BackgroundIn contrast to wild species, which have typically evolved phenotypes over long periods of natural selection, domesticates rapidly gained human-preferred agronomic traits in a relatively short-time frame via artificial selection. Under domesticated conditions, many traits can be observed that cannot only be due to environmental alteration. In the case of silkworms, aside from genetic divergence, whether epigenetic divergence played a role in domestication is an unanswered question. The silkworm is still an enigma in that it has two DNA methyltransferases (DNMT1 and DNMT2) but their functionality is unknown. Even in particular the functionality of the widely distributed DNMT1 remains unknown in insects in general.ResultsBy embryonic RNA interference, we reveal that knockdown of silkwormDnmt1caused decreased hatchability, providing the first direct experimental evidence of functional significance of insectDnmt1. In the light of this fact and those that DNA methylation is correlated with gene expression in silkworms and some agronomic traits in domesticated organisms are not stable, we comprehensively compare silk gland methylomes of 3 domesticated (Bombyx mori) and 4 wild (Bombyx mandarina) silkworms to identify differentially methylated genes between the two. We observed 2-fold more differentiated methylated cytosinces (mCs) in domesticated silkworms as compared to their wild counterparts, suggesting a trend of increasing DNA methylation during domestication. Further study of more domesticated and wild silkworms narrowed down the domesticates’ epimutations, and we were able to identify a number of differential genes. One such gene showing demethyaltion in domesticates correspondently displays lower gene expression, and more interestingly, has experienced selective sweep. A methylation-increased gene seems to result in higher expression in domesticates and the function of itsDrosophilahomolog was previously found to be essential for cell volume regulation, indicating a possible correlation with the enlargement of silk glands in domesticated silkworms.ConclusionsOur results imply epigenetic influences at work during domestication, which gives insight into long time historical controversies regarding acquired inheritance.
登录
查看更多内容
影响因子：
56.9
作者：
Jacobsen, SE;Meyerowitz, EM
通讯作者：
Meyerowitz, EM
影响因子：
8
作者：
Kim, J. K.;Samaranayake, M.;Pradhan, S.
通讯作者：
Pradhan, S.
影响因子：
64.5
作者：
Calarco JP;Borges F;Donoghue MT;Van Ex F;Jullien PE;Lopes T;Gardner R;Berger F;Feijó JA;Becker JD;Martienssen RA
通讯作者：
Martienssen RA
影响因子：
5.3
作者：
Biniszkiewicz, D;Gribnau, J;Jaenisch, R
通讯作者：
Jaenisch, R
DOI：
10.1073/pnas.0908755106
发表时间：
2009-11-24
影响因子：
11.1
作者：
Hauben, Miriam;Haesendonckx, Boris;De Block, Marc
通讯作者：
De Block, Marc