Optical reprogramming with ultrashort femtosecond laser pulses
Optical reprogramming with ultrashort femtosecond laser pulses
复制标题
使用超短飞秒激光脉冲进行光学重编程
作者：
A. Uchugonova;H. Breunig;A. Batista;K. König
亚15飞秒激光脉冲在干细胞研究中的使用进行了探索，特别强调体细胞的光学重编程。体细胞重编程为诱导多能干细胞（iPS）可以通过特定转录因子的异位表达来诱发。常规方法利用逆转录病毒/慢病毒来递送基因/转录因子以及促进转录因子整合到宿主基因组中。然而，病毒的使用可能导致由基因的随机整合引起的插入突变，因此，由于形成癌症的风险，这可能限制在临床应用中的使用。在这项研究中，一种新的方法是通过使用超短激光脉冲实现非病毒重编程，将转录因子引入细胞，从而产生iPS细胞。
The use of sub-15 femtosecond laser pulses in stem cell research is explored with particular emphasis on the optical reprogramming of somatic cells. The reprogramming of somatic cells into induced pluripotent stem (iPS) cells can be evoked through the ectopic expression of defined transcription factors. Conventional approaches utilize retro/lenti-viruses to deliver genes/transcription factors as well as to facilitate the integration of transcription factors into that of the host genome. However, the use of viruses may result in insertional mutations caused by the random integration of genes and as a result, this may limit the use within clinical applications due to the risk of the formation of cancer. In this study, a new approach is demonstrated in realizing non-viral reprogramming through the use of ultrashort laser pulses, to introduce transcription factors into the cell so as to generate iPS cells.
影响因子：
3.5
作者：
Mthunzi, Patience;Dholakia, Kishan;Gunn-Moore, Frank
通讯作者：
Gunn-Moore, Frank
DOI：
10.1073/pnas.87.20.7988
发表时间：
1990-10-01
影响因子：
11.1
作者：
CHOI, J;COSTA, ML;HOLTZER, H
通讯作者：
HOLTZER, H
超短飞秒激光微流控转染平台中人体细胞的光学重编程
影响因子：
2.8
作者：
Uchugonova;Breunig;Batista;König
通讯作者：
König