Optical reprogramming of human cells in an ultrashort femtosecond laser microfluidic transfection platform
Optical reprogramming of human cells in an ultrashort femtosecond laser microfluidic transfection platform
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超短飞秒激光微流控转染平台中人体细胞的光学重编程
DOI：
10.1002/jbio.201500240
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发表时间：
2015
影响因子：
2.8
通讯作者：
König
中科院分区：
文献类型：
--
作者：
Uchugonova;Breunig;Batista;König
诱导多能干细胞（iPS cell）技术可以用于产生无限数量的功能细胞，用于研究和治疗目的，而不会引起伦理争议。通常，病毒被用于有效的细胞内传递基因/转录因子以产生iPS细胞。然而，病毒基因组整合可能导致突变和肿瘤形成的风险，因此限制了其临床应用。在这里，我们证明了低于20飞秒的空间形状的极端超短激光脉冲诱导瞬态膜渗透，这使得在单个细胞水平上对微流控管中的细胞进行无污染转染具有多个基因，从而实现大细胞群的光学重编程。我们发现，超短飞秒激光微流控细胞转染平台在单次转染后提高了iPS样菌落形成的效率。用于生产iPS细胞集落的空间形状飞秒激光辅助微流控细胞转染平台示意图。
Induced pluripotent stem cell (iPS cell) technology can be used to produce unlimited numbers of functional cells for both research and therapeutic purposes without ethical controversy. Typically, viruses are applied for efficient intracellular delivery of genes/transcription factors to generate iPS cells. However, the viral genomic integration may cause a risk of mutation as well as tumor formation therefore limits its clinical application.Here we demonstrate that spatially shaped extreme ultrashort laser pulses of sub‐20 femtoseconds induce transient membrane permeabilisation which enables contamination‐free transfection of cells in a microfluidic tube with multiple genes at the individual cell level in order to achieve optical reprogramming of large cell populations. We found that the ultrashort femtosecond laser‐microfluidic cell transfection platform enhanced the efficacy of iPS‐like colony‐forming following merely a single transfection.Illustration of the spatially shaped femtosecond laser‐assisted microfluidic cell transfection platform for production of iPS cell colonies.
影响因子：
2.5
作者：
Breunig, Hans Georg;Uchugonova, Aisada;Koenig, Karsten
通讯作者：
Koenig, Karsten
探索哺乳动物细胞中膜通透性的超短脉冲激光参数空间。
影响因子：
4.6
作者：
Rudhall, Andrew P.;Antkowiak, Maciej;Tsampoula, Xanthi;Mazilu, Michael;Metzger, Nikolaus K.;Gunn-Moore, Frank;Dholakia, Kishan
通讯作者：
Dholakia, Kishan
影响因子：
64.5
作者：
Takahashi, Kazutoshi;Tanabe, Koji;Yamanaka, Shinya
通讯作者：
Yamanaka, Shinya