The Drosophila melanogaster Cajal body.

The Drosophila melanogaster Cajal body.
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DOI:
10.1083/jcb.200511038
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发表时间:
2006-03-13
期刊:
The Journal of cell biology
影响因子:
--
通讯作者:
Gall JG
Gall JG
中科院分区:
其他
文献类型:
--
作者:
Liu JL;Murphy C;Buszczak M;Clatterbuck S;Goodman R;Gall JG

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卡哈尔体(CB)是一种核细胞器,通常通过标记蛋白p80-线圈蛋白来识别。由于在果蝇中没有coilin的直向同源物,我们鉴定了D。黑腹CB使用其他成分的探针,相对诊断脊椎动物细胞中的CB。U85小CB特异性RNA、U2小核RNA、运动神经元存活蛋白和原纤维蛋白一起出现在与核仁密切相关的核体中。基于它与其他生物体中的CB的相似性,我们将这种结构称为D。黑腹藻令人惊讶的是,D。黑腹鱼U 7小核RNP存在于单独的核体中,我们称之为组蛋白基因座体(HLB)。HLB总是与组蛋白基因座共定位。因此,典型CB成分在D中至少分布在两个核体中。黑腹菌这些核体的鉴定现在允许广泛的问题被问及CB的结构和功能在遗传上易处理的有机体。
Cajal bodies (CBs) are nuclear organelles that are usually identified by the marker protein p80-coilin. Because no orthologue of coilin is known in Drosophila melanogaster, we identified D. melanogaster CBs using probes for other components that are relatively diagnostic for CBs in vertebrate cells. U85 small CB–specific RNA, U2 small nuclear RNA, the survival of motor neurons protein, and fibrillarin occur together in a nuclear body that is closely associated with the nucleolus. Based on its similarity to CBs in other organisms, we refer to this structure as the D. melanogaster CB. Surprisingly, the D. melanogaster U7 small nuclear RNP resides in a separate nuclear body, which we call the histone locus body (HLB). The HLB is invariably colocalized with the histone gene locus. Thus, canonical CB components are distributed into at least two nuclear bodies in D. melanogaster. The identification of these nuclear bodies now permits a broad range of questions to be asked about CB structure and function in a genetically tractable organism.
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发表时间: 1998-04-06
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