Induced Intermediate Mesoderm Combined with Decellularized Kidney Scaffolds for Functional Engineering Kidney
Induced Intermediate Mesoderm Combined with Decellularized Kidney Scaffolds for Functional Engineering Kidney
复制标题
诱导中间中胚层与脱细胞肾脏支架相结合用于肾脏功能工程
DOI：
10.1007/s13770-019-00197-9
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发表时间：
2019-06
影响因子：
3.6
通讯作者：
Zhao Shengtian
中科院分区：
文献类型：
--
作者：
Zhang Jianye;Li Kailin;Kong Feng;Sun Chao;Zhang Denglu;Yu Xin;Wang Xuesheng;Li Xian;Liu Tongyan;Shao Guangfeng;Guan Yong;Zhao Shengtian
慢性肾脏疾病是对人类健康的严重威胁，没有理想的治疗策略。成熟的哺乳动物肾脏具有固定数量的肾单位，一旦损坏，再生就很难。因此，开发一种有效的方法
BackgroundChronic kidney disease is a severe threat to human health with no ideal treatment strategy. Mature mammalian kidneys have a fixed number of nephrons, and regeneration is difficult once they are damaged. For this reason, developing an efficient approach to achieve kidney regeneration is necessary. The technology of the combination of decellularized kidney scaffolds with stem cells has emerged as a new strategy; however, in previous studies, the differentiation of stem cells in decellularized scaffolds was insufficient for functional kidney regeneration, and many problems remain.MethodsWe used 0.5% sodium dodecyl sulfate (SDS) to produce rat kidney decellularized scaffolds, and induce adipose-derived stem cells (ADSCs) into intermediate mesoderm by adding Wnt agonist CHIR99021 and FGF9in vitro. The characteristics of decellularized scaffolds and intermediate mesoderm induced from adipose–derived stem cells were identified. The scaffolds were recellularized with ADSCs and intermediate mesoderm cells through the renal artery and ureter. After cocultured for 10 days, cells adhesion and differentiation was evaluated.ResultsIntermediate mesoderm cells were successfully induced from ADSCs and identified by immunofluorescence and Western blotting assays (OSR1 + , PAX2 +). Immunofluorescence showed that intermediate mesoderm cells differentiated into tubular-like (E-CAD + , GATA3 +) and podocyte-like (WT1 +) cells with higher differentiation efficiency than ADSCs in the decellularized scaffolds. Comparatively, this phenomenon was not observed in induced intermediate mesoderm cells culturedin vitro.ConclusionIn this study, we demonstrated that intermediate mesoderm cells could be induced from ADSCs and that they could differentiate well after cocultured with decellularized scaffolds.
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影响因子：
2.2
作者：
Hinata, Nobuyuki;Suzuki, Ryoji;Fujisawa, Masato
通讯作者：
Fujisawa, Masato
影响因子：
4.3
作者：
Yamanaka S;Yokoo T
通讯作者：
Yokoo T
影响因子：
4
作者：
Perniconi B;Coletti D;Aulino P;Costa A;Aprile P;Santacroce L;Chiaravalloti E;Coquelin L;Chevallier N;Teodori L;Adamo S;Marrelli M;Tatullo M
通讯作者：
Tatullo M
影响因子：
4.6
作者：
Schmidt-Ott, Kai M.;Masckauchan, T. Nestor H.;Barasch, Jonathan
通讯作者：
Barasch, Jonathan
影响因子：
64.8
作者：
Takasato, Minoru;Er, Pei X.;Little, Melissa H.
通讯作者：
Little, Melissa H.