Investigating the molecular basis for heterophylly in the aquatic plant Potamogeton octandrus (Potamogetonaceae) with comparative transcriptomics.
Investigating the molecular basis for heterophylly in the aquatic plant Potamogeton octandrus (Potamogetonaceae) with comparative transcriptomics.
复制标题
通过比较转录组学研究水生植物眼子菜（眼子菜科）异源性的分子基础
作者：
He D;Guo P;Gugger PF;Guo Y;Liu X;Chen J
许多植物物种在一株植物内表现出不同的叶形态，或异叶。然而，调节这种现象的分子机制仍然难以捉摸。本研究利用高通量测序技术（RNA-Seq）对八蕊眼子菜（Potamogeton octandrus Poir）不同发育阶段的沉水叶和漂浮叶转录组进行了测序，以期为异叶性相关基因的发现和功能研究提供帮助。在沉水叶和漂浮叶中共鉴定出81，103个unigenes，通过比较不同发育时间点的样品，鉴定出6，822个差异表达基因（DEG）。KEGG途径富集分析将这些unigenes分为128条途径。共有24，025个差异表达基因涉及碳代谢途径、氨基酸生物合成、核糖体过程和植物-病原体相互作用。特别地，KEGG途径富集分析将总共70个DEG分类为植物激素信号转导途径。这里提出的高通量转录组学结果突出了理解heterophylly的分子机制的潜力，这仍然是知之甚少。此外，这些数据提供了一个框架，以更好地了解异形叶发育的八雄蕊通过有针对性的研究，利用基因克隆和功能分析。
Many plant species exhibit different leaf morphologies within a single plant, or heterophylly. The molecular mechanisms regulating this phenomenon, however, have remained elusive. In this study, the transcriptomes of submerged and floating leaves of an aquatic heterophyllous plant, Potamogeton octandrus Poir, at different stages of development, were sequenced using high-throughput sequencing (RNA-Seq), in order to aid gene discovery and functional studies of genes involved in heterophylly. A total of 81,103 unigenes were identified in submerged and floating leaves and 6,822 differentially expressed genes (DEGs) were identified by comparing samples at differing time points of development. KEGG pathway enrichment analysis categorized these unigenes into 128 pathways. A total of 24,025 differentially expressed genes were involved in carbon metabolic pathways, biosynthesis of amino acids, ribosomal processes, and plant-pathogen interactions. In particular, KEGG pathway enrichment analysis categorized a total of 70 DEGs into plant hormone signal transduction pathways. The high-throughput transcriptomic results presented here highlight the potential for understanding the molecular mechanisms underlying heterophylly, which is still poorly understood. Further, these data provide a framework to better understand heterophyllous leaf development in P. octandrus via targeted studies utilizing gene cloning and functional analyses.
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