Capturing and characterizing immune cells from breast tumor microenvironment: an innovative surgical approach.
Capturing and characterizing immune cells from breast tumor microenvironment: an innovative surgical approach.
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DOI：
10.1245/s10434-010-1029-9
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发表时间：
2010-10
影响因子：
3.7
通讯作者：
Mohamed MM
中科院分区：
文献类型：
--
作者：
El-Shinawi M;Abdelwahab SF;Sobhy M;Nouh MA;Sloane BF;Mohamed MM
在乳腺癌患者中，乳腺静脉引流液中可能含有具有免疫学重要性的细胞、正在播散的肿瘤细胞以及肿瘤微环境衍生的其他生物学因素。在改良乳房根治术期间和循环稀释前收集腋窝静脉血可以让我们确定肿瘤微环境的生物学特性。目的是：(1)发展一种通过腋窝静脉的分支从乳腺肿瘤微环境中采集血液的手术方法；(2)表征和比较从腋窝静脉采集的免疫细胞与乳腺癌患者外周血中的免疫细胞。我们招募了17名年龄在30-50岁之间的女性，通过乳房x光检查、超声检查和活检诊断为乳腺癌（II-III期）。所有患者术前均未接受治疗。在常规手术解剖中，从肝素管中采血，从腋窝静脉分支中采血10 mL，从外周血中采血10 mL。分离单个核细胞，流式细胞术测定不同白细胞群的百分比。我们检测到腋窝支脉收集的总T淋巴细胞和辅助T细胞的百分比显著增加，但与外周血相比，细胞毒性T细胞、单核细胞、自然杀伤细胞或B细胞的百分比没有显著增加。本研究验证了采用术中手术方法收集通过腋窝支流从肿瘤微环境中排出的白细胞。我们的研究结果显示，肿瘤微环境中总T淋巴细胞和T辅助细胞的浸润增加，表明它们可能参与肿瘤的发病机制。
In breast cancer patients, venous drainage of the breast may contain cells of immunological importance, tumor cells undergoing dissemination, and other biological factors derived from the tumor microenvironment. Collecting axillary venous blood during modified radical mastectomy and thus before dilution in the circulation may allow us to define biological properties of the tumor microenvironment. Aims were to (1) develop a surgical approach to collect blood from the breast tumor microenvironment through tributaries of the axillary vein and (2) characterize and compare immune cells collected from the axillary vein with those in peripheral blood of breast cancer patients. We enrolled 17 women aged 30–50 years and diagnosed with breast cancer by mammography, ultrasound, and biopsy (stages II–III). All patients were, preoperatively, treatment-naive. During routine surgical dissection, blood was collected in heparin tubes, 10 mL from tributaries of the axillary vein and 10 mL from peripheral blood. Mononuclear cells were separated, and percentages of different leukocyte populations were determined by flow cytometry. We detected a significant increase in the percentage of total T lymphocytes and T helper cells collected from axillary tributaries, but not in the percentages of cytotoxic T cells, monocytes, natural killer, or B cells compared with peripheral blood. The present study validated using an intra-operative surgical approach to collect leukocytes drained from the tumor microenvironment through axillary tributaries. Our results showed an increase in the infiltration of total T-lymphocytes and T helper cells in the tumor microenvironment, suggesting that they may contribute to tumor pathogenesis.
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DOI：
10.1084/jem.188.12.2357
发表时间：
1998-12-21
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Hung K;Hayashi R;Lafond-Walker A;Lowenstein C;Pardoll D;Levitsky H
通讯作者：
Levitsky H
腋窝淋巴结中免疫细胞的特征可以预测乳腺癌中无疾病的生存。
影响因子：
15.8
作者：
Kohrt, HE;Nouri, N;Nowels, K;Johnson, D;Holmes, S;Lee, PP
通讯作者：
Lee, PP
影响因子：
2.6
作者：
Denewer, Adel;Setit, Ahmed;Farouk, Omar
通讯作者：
Farouk, Omar
影响因子：
50.3
作者：
Allinen, M;Beroukhim, R;Polyak, K
通讯作者：
Polyak, K
影响因子：
11.5
作者：
Ladoire, Sylvain;Arnould, Laurent;Ghiringhelli, Francois
通讯作者：
Ghiringhelli, Francois