Genetic Evaluation and Population Structure of Jiangsu Native Pigs in China Revealed by SINE Insertion Polymorphisms.
Genetic Evaluation and Population Structure of Jiangsu Native Pigs in China Revealed by SINE Insertion Polymorphisms.
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SINE插入多态性揭示中国江苏土猪的遗传评价和群体结构
DOI：
10.3390/ani12111345
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发表时间：
2022-05-25
期刊：
影响因子：
3
通讯作者：
Song, Chengyi
中科院分区：
文献类型：
--
作者：
Wang, Xiaoyan;D'Alessandro, Enrico;Chi, Chenglin;Moawad, Ali Shoaib;Zong, Wencheng;Chen, Cai;Song, Chengyi
在前期的研究中，我们应用30个SINE-RIPs对7个中国小型猪群体进行了群体遗传分析，有效地证实了这些群体之间的遗传距离和品种关系。在中国东部的江苏省，有大量的以多产而闻名世界的土猪，如梅山、二花脸。由于猪肉生产依赖于有限的商业品种，如长白猪，大白色和杜洛克猪，其特点是在集约化生产系统中最大限度地提高生产力，这些本土猪现在急剧减少。这些资源的遗传特性是保护、选择和可持续利用的基本要求。因此，选择SINE-RIPs对江苏猪群体的遗传多样性和群体结构进行了分析，为江苏地方猪群体的保护和利用提供了依据。摘要短散在核元件（西内斯）是反转录转座子的一种，因其在基因组中分布广、拷贝数高、多态性高等特点，被认为是理想的分子标记。初步研究已经在猪基因组中发现了超过35，000个SINE-反转录转座子插入多态性（RIP）。本研究利用18个SINE-RIPs对江苏省7个地方猪群体和2个杂交猪群体的遗传变异和群体结构进行了分析。选择两个商品猪品种（杜洛克猪和大白色猪）和一个意大利本地品种（西西里黑猪）作为对照。结果表明，18个SINE-RIPs在猪群中均表现出多态性。根据期望杂合度和多态信息含量值，江苏本地猪群（二花脸、枫泾、梅山中猪、糜猪、沙屋头、梅山小猪和淮猪）的多态性高于杂交猪群（苏山猪和苏江猪）和外来猪群（西西里黑猪、大白色猪和杜洛克猪）。根据FIS值，小梅山猪、糜猪、梅山中猪和二花脸猪等地方猪的近交程度较高。在邻接树上，江苏本地猪群形成一个分支，而外来猪种形成另一个分支，其中两个杂交种的外来猪血统占50%以上。淮猪独立于其他江苏本地猪，但与苏姜和糜有共同的祖先。本研究结果为研究中国地方猪种的种群结构提供了新的视角，对中国地方猪的保护和利用具有一定的指导意义。
Simple Summary In a previous study, 30 SINE-RIPs were applied for population genetic analysis in 7 Chinese miniature pig populations and approved effectively in the genetic distances and breed-relationships between these populations. There are abundant indigenous pigs famous across the world for their prolificacy in the Jiangsu Province of eastern China, such as Meishan, Erhualian. Since pork production relies on limited commercial breeds such as Landrace, Large White, and Duroc pigs, characterized by maximizing productivity in intensive production systems, these indigenous pigs are nowadays decrease sharply. The genetic characterizations of these resources are essential requirements for the development of conservation, selection, and sustainable utilizations. Therefore, SINE-RIPs were selected to evaluate the genetic variation and population structure of Jiangsu pig populations and the results may assist with the conservation and utilization of these native pig populations. Abstract Short interspersed nuclear elements (SINEs), one type of retrotransposon, are considered to be ideal molecular markers due to their wide distribution in the genome, high copy number, and high polymorphism. Preliminary studies have identified more than 35,000 SINE-retrotransposon insertion polymorphisms (RIPs) in the pig genome. In this study, 18 SINE-RIPs were used to evaluate the genetic variation and population structure of seven native pig populations and two crossbreeds in the Jiangsu Province of China. Two commercial pig breeds (Duroc and Large White) and one Italian native breed (Sicilian Black pig) were selected as the control. The results showed that all 18 SINE-RIPs were polymorphic among these pigs. The Jiangsu native pig populations (Erhualian, Fengjing, Middle Meishan, Mi, Shawutou, Small Meishan, and Huai) were shown to be more polymorphic than the crossbreeds (Sushan and Sujiang) and external breeds (Sicilian Black pig, Large White, and Duroc) based on the expected heterozygosity and polymorphic information content values. Some native pigs, including Small Meishan, Mi, Middle Meishan, and Erhualian, had a higher degree of inbreeding according to the FIS values. Based on the neighbor-joining tree, all of the Jiangsu native pig populations formed one branch, while the three external pig breeds formed the other branches, with the two crossbreeds containing more than 50% external pig ancestry. The Huai pigs were independent of the other Jiangsu native pigs but shared a common ancestor with Sujiang and Mi. The results provide a new perspective on the population structure of these native pig breeds and will assist with the conservation and utilization of Chinese native pigs.
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DOI：
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发表时间：
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期刊：
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影响因子：
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