The NPR1-dependent salicylic acid signalling pathway is pivotal for enhanced salt and oxidative stress tolerance in Arabidopsis.
The NPR1-dependent salicylic acid signalling pathway is pivotal for enhanced salt and oxidative stress tolerance in Arabidopsis.
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DOI：
10.1093/jxb/eru528
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发表时间：
2015-04
影响因子：
6.9
通讯作者：
Rengel Z
中科院分区：
文献类型：
--
作者：
Jayakannan M;Bose J;Babourina O;Shabala S;Massart A;Poschenrieder C;Rengel Z
NPR1依赖的水杨酸信号通路在盐和氧化胁迫下通过去极化激活的外向整流钾和ROS激活的非选择性阳离子通道控制钠进入根，同时防止钾的损失。内源水杨酸(SA)信号通路在植物对盐和氧化胁迫的反应中的作用尚不清楚。在盐胁迫或氧化胁迫下，将拟南芥SA信号转导突变体npr1-5(病程相关基因1不表达)和nudt7(Nudex Hylase7)与野生型(Col-0)进行了比较。生长和活力检测表明，与野生型相比，npr1-5突变体对盐胁迫或氧化胁迫敏感，而nudt7突变体对盐胁迫和氧化胁迫敏感。与野生型和nudt7突变体相比，急性盐胁迫引起npr1-5根最强的膜电位去极化，最高的钠和质子内流，以及钾的损失。虽然盐胁迫诱导的过氧化氢产量在npr1-5突变体中最低，但活性氧物种(ROS)胁迫(由1 mM产生羟基自由基的铜-抗坏血酸混合物或1或10 mM过氧化氢诱导)导致npr1-5突变体的根中钾流失高于野生型和nudt7突变体。与野生型和nudt7突变体相比，长期盐胁迫导致npr1-5突变体的地上部钠含量最高，钾含量最低。上述结果表明，依赖于NPR1的SA信号在(I)控制Na+进入根组织和随后的长距离运输到地上部，以及(Ii)通过去极化激活的外向整流钾和ROS激活的非选择性阳离子通道来防止钾损失起着关键作用。综上所述，依赖于NPR1的SA信号对拟南芥的耐盐性和氧化胁迫是至关重要的。
NPR1-dependent salicylic acid signalling controls sodium entry into the roots while preventing potassium loss through depolarization-activated outward-rectifying potassium and ROS-activated non-selective cation channels during salt and oxidative stresses. The role of endogenous salicylic acid (SA) signalling cascades in plant responses to salt and oxidative stresses is unclear. Arabidopsis SA signalling mutants, namely npr1-5 (non-expresser of pathogenesis related gene1), which lacks NPR1-dependent SA signalling, and nudt7 (nudix hydrolase7), which has both constitutively expressed NPR1-dependent and NPR1-independent SA signalling pathways, were compared with the wild type (Col-0) during salt or oxidative stresses. Growth and viability staining showed that, compared with wild type, the npr1-5 mutant was sensitive to either salt or oxidative stress, whereas the nudt7 mutant was tolerant. Acute salt stress caused the strongest membrane potential depolarization, highest sodium and proton influx, and potassium loss from npr1-5 roots in comparison with the wild type and nudt7 mutant. Though salt stress-induced hydrogen peroxide production was lowest in the npr1-5 mutant, the reactive oxygen species (ROS) stress (induced by 1mM of hydroxyl-radical-generating copper-ascorbate mix, or either 1 or 10mM hydrogen peroxide) caused a higher potassium loss from the roots of the npr1-5 mutant than the wild type and nudt7 mutant. Long-term salt exposure resulted in the highest sodium and the lowest potassium concentration in the shoots of npr1-5 mutant in comparison with the wild type and nudt7 mutant. The above results demonstrate that NPR1-dependent SA signalling is pivotal to (i) controlling Na+ entry into the root tissue and its subsequent long-distance transport into the shoot, and (ii) preventing a potassium loss through depolarization-activated outward-rectifying potassium and ROS-activated non-selective cation channels. In conclusion, NPR1-dependent SA signalling is central to the salt and oxidative stress tolerance in Arabidopsis.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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