A WRKY transcription factor, TaWRKY42-B, facilitates initiation of leaf senescence by promoting jasmonic acid biosynthesis.
A WRKY transcription factor, TaWRKY42-B, facilitates initiation of leaf senescence by promoting jasmonic acid biosynthesis.
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WRKY 转录因子 TaWRKY42-B 通过促进茉莉酸生物合成促进叶片衰老的启动
DOI：
10.1186/s12870-020-02650-7
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发表时间：
2020-09-29
影响因子：
5.3
通讯作者：
Zhou CJ
中科院分区：
文献类型：
--
作者：
Zhao MM;Zhang XW;Liu YW;Li K;Tan Q;Zhou S;Wang G;Zhou CJ
叶片衰老包括许多协同事件，将环境信号与年龄相关的发育线索整合在一起，协调营养的多方面退化和源库分配。在作物中，叶片衰老一直被认为是产量和品质的重要发育阶段，而参与调控叶片衰老的候选基因的功能特性在小麦中迄今还很有限。在这项研究中，我们分析了97个WRKY转录因子(TF)在小麦叶片衰老过程中的表达谱，并随后分离到一个潜在的叶片衰老调节因子TaWRKY42-B，用于进一步的功能研究。通过对TaWRKY42-B高表达的拟南芥植株和TaWRKY42-B沉默的小麦植株的表型和生理分析，我们证实了TaWRKY42-B在启动发育和暗诱导叶片衰老中的积极作用。此外，我们的结果表明，TaWRKY42-B主要通过与茉莉酸生物合成基因AtLOX3及其同源基因TaLOX3相互作用来促进叶片衰老，从而促进茉莉酸含量的积累。在本研究中，我们还证明了TaWRKY42-B与AtWRKY53在启动与年龄相关的叶片衰老过程中具有功能保守性。我们的结果揭示了一个新的叶片衰老正调控基因TaWRKY42-B，它通过激活茉莉酸的生物合成来调节与茉莉酸相关的叶片衰老，有可能成为小麦分子育种的目标基因。
Leaf senescence comprises numerous cooperative events, integrates environmental signals with age-dependent developmental cues, and coordinates the multifaceted deterioration and source-to-sink allocation of nutrients. In crops, leaf senescence has long been regarded as an essential developmental stage for productivity and quality, whereas functional characterization of candidate genes involved in the regulation of leaf senescence has, thus far, been limited in wheat. In this study, we analyzed the expression profiles of 97 WRKY transcription factors (TFs) throughout the progression of leaf senescence in wheat and subsequently isolated a potential regulator of leaf senescence, TaWRKY42-B, for further functional investigation. By phenotypic and physiological analyses in TaWRKY42-B-overexpressing Arabidopsis plants and TaWRKY42-B-silenced wheat plants, we confirmed the positive role of TaWRKY42-B in the initiation of developmental and dark-induced leaf senescence. Furthermore, our results revealed that TaWRKY42-B promotes leaf senescence mainly by interacting with a JA biosynthesis gene, AtLOX3, and its ortholog, TaLOX3, which consequently contributes to the accumulation of JA content. In the present study, we also demonstrated that TaWRKY42-B was functionally conserved with AtWRKY53 in the initiation of age-dependent leaf senescence. Our results revealed a novel positive regulator of leaf senescence, TaWRKY42-B, which mediates JA-related leaf senescence via activation of JA biosynthesis and has the potential to be a target gene for molecular breeding in wheat.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
5.6
作者：
Gao, Huiming;Wang, Yafei;Zhang, Zhengbin
通讯作者：
Zhang, Zhengbin
影响因子：
7.2
作者：
Ay, Nicole;Irmler, Kristina;Humbeck, Klaus
通讯作者：
Humbeck, Klaus
拟南芥 WRKY45 与 DELLA 蛋白 RGL1 相互作用，积极调节年龄引发的叶片衰老
影响因子：
27.5
作者：
Chen, Ligang;Xiang, Shengyuan;Yu, Diqiu
通讯作者：
Yu, Diqiu
影响因子：
4.6
作者：
Jaemsaeng R;Jantasuriyarat C;Thamchaipenet A
通讯作者：
Thamchaipenet A