Rapid and reliable quantitation of amino acids and myo-inositol in mouse brain by high performance liquid chromatography and tandem mass spectrometry.
Rapid and reliable quantitation of amino acids and myo-inositol in mouse brain by high performance liquid chromatography and tandem mass spectrometry.
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DOI：
10.1016/j.jchromb.2012.01.035
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发表时间：
2012-04-15
影响因子：
3
通讯作者：
Alnouti, Yazen
中科院分区：
文献类型：
--
作者：
Bathena, Sai P.;Huang, Jiangeng;Epstein, Adrian A.;Gendelman, Howard E.;Boska, Michael D.;Alnouti, Yazen
氨基酸和肌醇长期以来被认为是神经退行性疾病的潜在生物标志物。准确的测量和稳定性问题阻碍了它们的选择性使用。为此，开发了一种基于多反应监测的灵敏液相色谱 - 串联质谱（LC - MS/MS）方法，通过甲醇萃取同时对小鼠大脑中的谷氨酰胺、谷氨酸、γ - 氨基丁酸（GABA）、天冬氨酸、N - 乙酰天冬氨酸、牛磺酸、胆碱、肌酸、磷酸胆碱和肌醇进行定量。采用亲水相互作用色谱硅胶柱进行色谱分析，总运行时间为15分钟。经验证，该方法具有选择性、灵敏性、准确性和精确性。对于一系列分析物，该方法的定量限为2.5至20纳克/毫升，动态范围为2.5 - 20至500 - 4000纳克/毫升。这种LC - MS/MS方法已在人类神经系统疾病模型中进行了生物标志物发现的验证。
Amino acids and myo-inositol have long been proposed as putative biomarkers for neurodegenerative diseases. Accurate measures and stability have precluded their selective use. To this end, a sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method based on multiple reaction monitoring was developed to simultaneously quantify glutamine, glutamate, γ-aminobutyric acid (GABA), aspartic acid, N-acetyl aspartic acid, taurine, choline, creatine, phosphocholine and myo-inositol in mouse brain by methanol extractions. Chromatography was performed using a hydrophilic interaction chromatography silica column within in a total run time of 15 min. The validated method is selective, sensitive, accurate, and precise. The method has a limit of quantification ranging from 2.5 to 20 ng/ml for a range of analytes and a dynamic range from 2.5–20 to 500–4000 ng/ml. This LC-MS/MS method was validated for biomarker discovery in models of human neurological disorders.
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DOI：
10.1016/s0021-9673(00)96972-3
发表时间：
1990-01-19
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
ALPERT, AJ
通讯作者：
ALPERT, AJ
影响因子：
4.7
作者：
Rissman RA;Mobley WC
通讯作者：
Mobley WC
DOI：
10.1083/jcb.200207113
发表时间：
2003-01-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ehehalt R;Keller P;Haass C;Thiele C;Simons K
通讯作者：
Simons K
影响因子：
3
作者：
Buck, Kerstin;Voehringer, Patrizia;Ferger, Boris
通讯作者：
Ferger, Boris
影响因子：
7.4
作者：
Gu, Liping;Jones, A. Daniel;Last, Robert L.
通讯作者：
Last, Robert L.