The Malus domestica sugar transporter gene family: identifications based on genome and expression profiling related to the accumulation of fruit sugars.
The Malus domestica sugar transporter gene family: identifications based on genome and expression profiling related to the accumulation of fruit sugars.
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DOI：
10.3389/fpls.2014.00569
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发表时间：
2014
影响因子：
5.6
通讯作者：
Li M
中科院分区：
文献类型：
--
作者：
Wei X;Liu F;Chen C;Ma F;Li M
在植物中，糖转运蛋白不仅参与糖的长距离运输，还参与糖在汇细胞中的积累。为了鉴定糖转运基因家族成员并分析其在果实糖积累中的功能，我们对家苹果基因组进行了系统发育分析。以“Gala”苹果的茎尖、成熟叶片和发育果实为研究对象进行了表达谱分析。糖醇基因[包括17个山梨醇转运蛋白（SOTs）]、蔗糖和单糖转运蛋白以及SWEET基因分别在基因组的31个、9个、50个和27个位点中被选为候选基因。单糖转运蛋白家族似乎包括5个亚家族（30个MdHTs， 8个MdEDR6s， 5个MdTMTs， 3个MdvGTs和4个MdpGLTs）。系统发育分析表明，苹果、葡萄和拟南芥之间存在同源基因。转录本的研究表明，68个候选转运蛋白在苹果中表达，尽管表达程度不同。在这里，我们根据它们的表达水平和糖浓度之间的关系来讨论它们可能的作用。苹果果实中果糖的高积累可能与MdTMT1/2和MdEDR6之间的协调合作有关。相比之下，这些水果显示MdSWEET4.1的低表达和山梨醇产生的果糖的高通量。我们的研究对糖转运基因进行了详尽的调查，并证明家蝇的糖转运基因家族与其他物种的相似。这些转运蛋白的表达谱分析可能有助于提高我们对它们在果实形成和甜味特性发展中的生理功能的理解。
In plants, sugar transporters are involved not only in long-distance transport, but also in sugar accumulations in sink cells. To identify members of sugar transporter gene families and to analyze their function in fruit sugar accumulation, we conducted a phylogenetic analysis of the Malus domestica genome. Expression profiling was performed with shoot tips, mature leaves, and developed fruit of “Gala” apple. Genes for sugar alcohol [including 17 sorbitol transporters (SOTs)], sucrose, and monosaccharide transporters, plus SWEET genes, were selected as candidates in 31, 9, 50, and 27 loci, respectively, of the genome. The monosaccharide transporter family appears to include five subfamilies (30 MdHTs, 8 MdEDR6s, 5 MdTMTs, 3 MdvGTs, and 4 MdpGLTs). Phylogenetic analysis of the protein sequences indicated that orthologs exist among Malus, Vitis, and Arabidopsis. Investigations of transcripts revealed that 68 candidate transporters are expressed in apple, albeit to different extents. Here, we discuss their possible roles based on the relationship between their levels of expression and sugar concentrations. The high accumulation of fructose in apple fruit is possibly linked to the coordination and cooperation between MdTMT1/2 and MdEDR6. By contrast, these fruits show low MdSWEET4.1 expression and a high flux of fructose produced from sorbitol. Our study provides an exhaustive survey of sugar transporter genes and demonstrates that sugar transporter gene families in M. domestica are comparable to those in other species. Expression profiling of these transporters will likely contribute to improving our understanding of their physiological functions in fruit formation and the development of sweetness properties.
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影响因子：
4.8
作者：
Carpaneto, A;Geiger, D;Hedrich, R
通讯作者：
Hedrich, R
影响因子：
6.9
作者：
Antony, Edna;Taybi, Tahar;Borland, Anne M.
通讯作者：
Borland, Anne M.
影响因子：
4.9
作者：
Poschet, Gernot;Hannich, Barbara;Buettner, Michael
通讯作者：
Buettner, Michael
影响因子：
5.3
作者：
Afoufa-Bastien D;Medici A;Jeauffre J;Coutos-Thévenot P;Lemoine R;Atanassova R;Laloi M
通讯作者：
Laloi M
影响因子：
27.5
作者：
McCurdy, David W.;Dibley, Stephen;Patrick, John W.
通讯作者：
Patrick, John W.