The Vitis vinifera sugar transporter gene family: phylogenetic overview and macroarray expression profiling.
The Vitis vinifera sugar transporter gene family: phylogenetic overview and macroarray expression profiling.
复制标题
DOI：
10.1186/1471-2229-10-245
复制
发表时间：
2010-11-12
影响因子：
5.3
通讯作者：
Laloi M
中科院分区：
文献类型：
--
作者：
Afoufa-Bastien D;Medici A;Jeauffre J;Coutos-Thévenot P;Lemoine R;Atanassova R;Laloi M
在较高的植物中，糖不仅是养分，而且是重要的信号分子。它们是通过糖转运蛋白通过植物分布的，糖转运蛋白不仅参与了通过负载和卸载导电复合物的糖长距离运输，而且还参与了糖分分配到源和水槽细胞中。最近发布的葡萄干基因组序列的可用性为木质物种中鉴定蔗糖和单糖转运蛋白基因家族提供了机会，并使用系统发育分析将它们与草本拟南芥的含量进行比较。
在葡萄藤中，世界上最重要的水果作物之一，似乎分别存在于4和59个基因座中的蔗糖和单糖转运蛋白基因，并且单糖转运蛋白转运蛋白家族可以分为7个亚家族。蛋白质序列的系统发育分析表明，葡萄和拟南芥之间存在直系同源物。搜索最有特征性的转运蛋白基因家族（蔗糖，己糖和多醇转运蛋白）的启动子序列中的顺式调节元件，表明其中一些可能受糖调节。为了同时介绍几个基因，我们创建了一个特有20个糖转运蛋白基因的cDNA片段的宏观阵列。这种宏观阵列分析表明，在大多数营养器官中，高度表达了两个己糖（VVHT1，VVHT3），一个（VVPMT5）和一个蔗糖（VVSUC27）转运蛋白基因。在浆果发育过程中，调节了一个己糖转运蛋白（VVHT2）和两个构成塑料单糖转运蛋白（VVTMT1，VVTMT2）基因的表达。最后，三个假定的己糖转运蛋白基因显示出在种子（VVHT3，VVHT5），根（VVHT2）或成熟叶片（VVHT5）中高度表达的优先器官特异性。
这项研究提供了对Vitis Vinifera中糖转运蛋白基因的详尽调查，并揭示了这种木本植物中的糖转运蛋白基因家族与草本植物的糖基因家族相当。专用的宏观阵列提供了葡萄糖糖转运蛋白基因的表达分析，这可能有助于了解其在植物和浆果发育中的生理功能。目前的结果可能还会对我们对植物糖转运蛋白的知识产生重大影响。
In higher plants, sugars are not only nutrients but also important signal molecules. They are distributed through the plant via sugar transporters, which are involved not only in sugar long-distance transport via the loading and the unloading of the conducting complex, but also in sugar allocation into source and sink cells. The availability of the recently released grapevine genome sequence offers the opportunity to identify sucrose and monosaccharide transporter gene families in a woody species and to compare them with those of the herbaceous Arabidopsis thaliana using a phylogenetic analysis. In grapevine, one of the most economically important fruit crop in the world, it appeared that sucrose and monosaccharide transporter genes are present in 4 and 59 loci, respectively and that the monosaccharide transporter family can be divided into 7 subfamilies. Phylogenetic analysis of protein sequences has indicated that orthologs exist between Vitis and Arabidospis. A search for cis-regulatory elements in the promoter sequences of the most characterized transporter gene families (sucrose, hexoses and polyols transporters), has revealed that some of them might probably be regulated by sugars. To profile several genes simultaneously, we created a macroarray bearing cDNA fragments specific to 20 sugar transporter genes. This macroarray analysis has revealed that two hexose (VvHT1, VvHT3), one polyol (VvPMT5) and one sucrose (VvSUC27) transporter genes, are highly expressed in most vegetative organs. The expression of one hexose transporter (VvHT2) and two tonoplastic monosaccharide transporter (VvTMT1, VvTMT2) genes are regulated during berry development. Finally, three putative hexose transporter genes show a preferential organ specificity being highly expressed in seeds (VvHT3, VvHT5), in roots (VvHT2) or in mature leaves (VvHT5). This study provides an exhaustive survey of sugar transporter genes in Vitis vinifera and revealed that sugar transporter gene families in this woody plant are strongly comparable to those of herbaceous species. Dedicated macroarrays have provided a Vitis sugar transporter genes expression profiling, which will likely contribute to understand their physiological functions in plant and berry development. The present results might also have a significant impact on our knowledge on plant sugar transporters.
登录
查看更多内容
影响因子：
4.4
作者：
Deluc LG;Grimplet J;Wheatley MD;Tillett RL;Quilici DR;Osborne C;Schooley DA;Schlauch KA;Cushman JC;Cramer GR
通讯作者：
Cramer GR
受糖调节的葡萄假定己糖转运基因的表达
影响因子：
7.4
作者：
Atanassova, R;Leterrier, M;Delrot, S
通讯作者：
Delrot, S
影响因子：
7.2
作者：
Acevedo-Hernández, GJ;León, P;Herrera-Estrella, LR
通讯作者：
Herrera-Estrella, LR
影响因子：
14.9
作者：
Dereeper A;Guignon V;Blanc G;Audic S;Buffet S;Chevenet F;Dufayard JF;Guindon S;Lefort V;Lescot M;Claverie JM;Gascuel O
通讯作者：
Gascuel O
影响因子：
6.5
作者：
Anisimova, Maria;Gascuel, Olivier
通讯作者：
Gascuel, Olivier