Screening and identification of human ZnT8-specific single-chain variable fragment (scFv) from type 1 diabetes phage display library
Screening and identification of human ZnT8-specific single-chain variable fragment (scFv) from type 1 diabetes phage display library
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从1型糖尿病噬菌体展示库中筛选和鉴定人ZnT8特异性单链可变片段（scFv）
DOI：
10.1007/s11427-016-5077-7
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发表时间：
2016-06
影响因子：
9.1
通讯作者：
Zhang Mei
中科院分区：
文献类型：
--
作者：
Wu Qian;Wang Xiaodong;Gu Yong;Zhang Xiao;Qin Yao;Chen Heng;Xu Xinyu;Yang Tao;Zhang Mei
Zinc transporter 8 (ZnT8) is a major autoantigen and a predictive marker in type 1 diabetes (T1D). To investigate ZnT8-specific antibodies, a phage display library from T1D was constructed and single-chain antibodies against ZnT8 were screened and identified. Human T1D single-chain variable fragment (scFv) phage display library consists of approximately 1×108clones. After four rounds of bio-panning, seven unique clones were positive by phage ELISA. Among them, C27 and C22, which demonstrated the highest affinity to ZnT8, were expressed inEscherichia coliTop10F’ and then purified by affinity chromatography. C27 and C22 specifically bound ZnT8 N/C fusion protein and ZnT8 C terminal dimer with one Arg325Trp mutation. The specificity to human islet cells of these scFvs were further confirmed by immunohistochemistry. In conclusion, we have successfully constructed a T1D phage display antibody library and identified two ZnT8-specific scFv clones, C27 and C22. These ZnT8-specific scFvs are potential agents in immunodiagnostic and immunotherapy of T1D.
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影响因子：
4.8
作者：
Weijers RN
通讯作者：
Weijers RN
影响因子：
7
作者：
Brenner MK;Heslop HE
通讯作者：
Heslop HE
DOI：
--
发表时间：
1980-03
期刊：
Annals of allergy
影响因子：
--
作者：
M. Richter;F. Daquillard
通讯作者：
M. Richter;F. Daquillard
DOI：
10.1073/pnas.0705894104
发表时间：
2007-10-23
影响因子：
11.1
作者：
Wenzlau, Janet M.;Juhl, Kirstine;Hutton, John C.
通讯作者：
Hutton, John C.
识别保守 HA1 表位的人 4F5 单链 Fv 抗体对不同分支的 H5N1 甲型流感病毒具有广泛的中和效力
影响因子：
7.6
作者：
Zhang，Xiao;Qi，Xian;Jiao，Yongjun
通讯作者：
Jiao，Yongjun