Heat studies with human IgE: A simple method for rapidly distinguishing sera with high total IgE levels
Heat studies with human IgE: A simple method for rapidly distinguishing sera with high total IgE levels
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人类 IgE 热研究：快速区分总 IgE 水平高的血清的简单方法
DOI：
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发表时间：
1974
期刊：
影响因子：
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通讯作者：
J. Pepys
中科院分区：
文献类型：
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作者：
B. Baldo;I. Wells;J. Pepys
对涵盖广泛总IgE浓度范围的人血清进行不同时间段的温和热处理，然后检查其与Fc特异性放射性标记的兔抗人IgE血清结合的能力。作为热处理的结果，发生血清IgE的聚集，并且聚集的材料与标记的抗血清显著反应。用标准人IgE血清和100多份来自正常和过敏患者的不同人血清样品进行的实验表明，这种简单的热方法可以区分血清中的高、中、低水平IgE。只要进行充分的检查，该方法提供了一种快速检查大量血清样品并区分IgE水平升高的血清的简单方法。
Human sera, covering a wide range of total IgE concentrations, were subjected to mild heat treatment for varying periods of time and then examined for their abilities to bind to an Fc‐specific, radioactively labelled rabbit anti‐human IgE serum. As a result of the heat treatment, aggregation of the serum IgE occurred and the aggregated material reacted significantly with the labelled antiserum. Experiments with a standard human IgE serum and over 100 different human serum samples from normal and allergic patients showed that this simple heat method can distinguish high, medium and low levels of IgE in serum. Provided that sufficient examinations are undertaken, the method offers a simple means of rapidly examining large numbers of serum samples and distinguishing those sera with elevated levels of IgE.
影响因子：
2
作者：
Liu, Yanming;Yue, Haiyan;Li, Furong
通讯作者：
Li, Furong