Loss of LIMCH1 predicts poor prognosis in patients with surgically resected Lung Adenocarcinoma: A study based on Immunohistochemical Analysis and Bioinformatics.
Loss of LIMCH1 predicts poor prognosis in patients with surgically resected Lung Adenocarcinoma: A study based on Immunohistochemical Analysis and Bioinformatics.
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LIMCH1 缺失预示手术切除肺腺癌患者预后不良：一项基于免疫组织化学分析和生物信息学的研究。
DOI：
10.7150/jca.47883
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发表时间：
2021
影响因子：
3.9
通讯作者：
Zhou J
中科院分区：
文献类型：
--
作者：
Cao H;Zhao J;Chen Z;Sun W;Ruan K;Zhou J;Zhou J
背景资料：LIMCH 1是一种新的肌动蛋白结合蛋白，据报道与多种癌症类型的肿瘤发生相关，但其在肺腺癌（LUAD）患者中的临床预后价值尚不清楚。方法：对196例LUAD患者行R 0切除术进行分析。采用免疫组化和数据挖掘方法检测肿瘤标本中LIMCH 1的表达，卡方检验分析LUAD患者LIMCH 1表达与临床病理因素的相关性，Kaplan-Meier曲线和考克斯比例风险模型分析LUAD患者LIMCH 1表达的临床预后作用。大卫富集和基因集富集分析（GSEA）用于确定潜在的分子机制。结果：LUAD组织中LIMCH 1蛋白和mRNA表达均明显降低。LIMCH 1 mRNA表达是TCGA队列中潜在的诊断指标，并与不良预后相关。我们的LUAD队列的IHC结果表明，LIMCH 1表达水平与胸膜浸润、肿瘤长度、肿瘤分化级别和临床肿瘤分期显著相关。LIMCH 1高表达的患者总体生存时间较长。考克斯多变量生存分析显示LIMCH 1表达独立预测预后。GO和KEGG聚类分析表明，LIMCH 1相关基因可能参与“细胞粘附”，“信号转导”和几个癌症相关的途径。GSEA在LIMCH 1低表达组中显示出8个富集标志，包括mTOR信号传导、MYC信号传导、DNA修复和G2 M检查点。结论：我们的研究结果表明，LIMCH 1可能作为一个有前途的生物标志物，预测LUAD的预后。
Background: LIMCH1, a novel actin-binding protein, is reported to correlate with tumorigenesis in multiple cancer types, but its clinical prognostic value in lung adenocarcinoma (LUAD) patients remains unclear. Methods: A total of 196 patients with LUAD who underwent R0 resection were included for analysis. We integrated immunohistochemistry (IHC) and data mining analyses to determine LIMCH1 expression in tumor specimens; the chi-square test was used to explore the correlation between clinicopathologic factors and LIMCH1 expression in LUAD; Kaplan-Meier curves and the Cox proportional hazards model were used to investigate the clinical prognostic role of LIMCH1 expression in patients with LUAD; and DAVID enrichment and gene set enrichment analysis (GSEA) were used to determine the underlying molecular mechanism. Results: LIMCH1 protein and mRNA expressions were significantly decreased in LUAD tissues. LIMCH1 mRNA expression was a potential diagnostic indicator in the TCGA cohort, and was associated with poor prognosis. IHC results in our LUAD cohort demonstrated that the LIMCH1 expression level was significantly associated with pleural invasion, tumor length, tumor differentiation grade, and clinical tumor stage. Patients with higher LIMCH1 expression had longer overall survival times. Cox multivariate survival analysis showed that LIMCH1 expression independently predicted the outcome. GO and KEGG clustering analyses showed that LIMCH1-related genes may be involved in 'cell adhesion', 'signal transduction', and several cancer-related pathways. GSEA showed 8 enriched hallmarks in the low LIMCH1 expression group, including mTOR signaling, MYC signaling, DNA repair, and G2M checkpoint. Conclusions: Our findings suggest that LIMCH1 may serve as a promising biomarker to predict LUAD prognosis.
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影响因子：
7.3
作者：
Gao J;Aksoy BA;Dogrusoz U;Dresdner G;Gross B;Sumer SO;Sun Y;Jacobsen A;Sinha R;Larsson E;Cerami E;Sander C;Schultz N
通讯作者：
Schultz N
影响因子：
3.3
作者：
Lin YH;Zhen YY;Chien KY;Lee IC;Lin WC;Chen MY;Pai LM
通讯作者：
Pai LM
影响因子：
12.3
作者：
Adler P;Kolde R;Kull M;Tkachenko A;Peterson H;Reimand J;Vilo J
通讯作者：
Vilo J
影响因子：
5.8
作者：
Li, Shau-Hsuan;Lu, Hung-I;Chen, Chang-Han
通讯作者：
Chen, Chang-Han
ANKS1B是透明细胞肾细胞癌中与吸烟相关的分子改变。
影响因子：
2
作者：
Eckel-Passow JE;Serie DJ;Bot BM;Joseph RW;Cheville JC;Parker AS
通讯作者：
Parker AS