Transcripts and low nitrogen tolerance: Regulatory and metabolic pathways in sugarcane under low nitrogen stress
Transcripts and low nitrogen tolerance: Regulatory and metabolic pathways in sugarcane under low nitrogen stress
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转录本和低氮耐受性：低氮胁迫下甘蔗的调控和代谢途径
DOI：
10.1016/j.envexpbot.2019.04.010
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发表时间：
2019-07
影响因子：
5.7
通讯作者：
Liping Xu
中科院分区：
文献类型：
--
作者：
Yingying Yang;Shiwu Gao;Yachun Su;Zhaoli Lin;Jinlong Guo;Mingjie Li;Zhoutao Wang;Youxiong Que;Liping Xu
了解甘蔗生理特性的分子调控机制对提高甘蔗耐低氮性至关重要。选用耐低氮品种ROC22和低氮敏感品种巴迪拉作为试验材料，ROC22的植株鲜重和植株氮积累N敏感性指标分别比巴迪拉低38.18%和17.44%。根据形态学和生理测量以及8个氮代谢关键基因的表达，选择低氮（0.6mMN）条件下ROC22和Badila的叶片（6h）和根系（3h）进行RNA-Seq分析。在叶片和根系中，ROC22的差异表达基因（DEGs）总数和上调数量均显著高于Badila。ROC22叶片中的特异deg主要富集于光合作用和N代谢，而ROC22根系中的特异deg主要富集于N代谢和激素信号转导途径。在两个品种中，MYB是最大的差异表达转录因子（TF）基因家族，其次是AP2-EREBP。在ROC22叶片和根系中，与IAA和ABA激素通路、N同化过程以及光合途径的PSI和PSII相关的几个基因调控了低氮胁迫下的生理特性，IAA浓度（叶片）和9.24%（根系）分别增加了11.47%和9.24%，谷氨酰胺合成酶（GS）酶活性（叶片）和36.97%（根系）分别增加了28.13%和36.97%，这可能解释了ROC22与巴地拉在低氮胁迫下的差异。本研究为甘蔗适应低氮条件的改良提供了依据。
Understanding the molecular regulatory mechanism of physiological characteristicsis essential to improve low nitrogen(N ) tolerance in sugarcane. We selected sugarcane varieties ROC22 (lowN-tolerantvariety) and Badila (low N-sensitive variety) as experimental materials, and N sensitivity indexes (NSIs) of plant fresh weight and plant N accumulation in ROC22 was respectively 38.18% and 17.44% lower than that of Badila. Based on the morphological and physiological measurements and the expression of eight key genes in N metabolism, the leaves (6h) and roots (3h) of ROC22 and Badila under low N condition (0.6mMN) were selected for RNA-Seq analysis. In the leaves and roots, the total and up-regulated numbers of differentially expressedgenes (DEGs) in ROC22 was significantly higher than that of Badila.The specific DEGs in the ROC22 leaves mainly enriched in photosynthesis and N metabolism, whereas those in the ROC22 roots mainly enriched in N metabolism and the hormone signal transduction pathway. MYB was the largest differentially expressed transcription factor (TF) gene family in both varieties, followed by AP2-EREBP.In the leaves and roots of ROC22, several genes related to IAA and ABA hormone pathways, N assimilation process, and PSI and PSII of the photosynthesis pathways modulated the physiological characteristics under low N stress, correspondingly, IAA concentration increased by 11.47% (leaves) and 9.24% (roots), and glutamine synthetase (GS) enzyme activities increased by 28.13% (leaves) and 36.97% (roots), which may explain the observed differences in low N tolerance between ROC22 and Badila. This study has provided a basis for improvement of sugarcane in adapting to low N conditions..
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转录组测序揭示了转录因子在玉米中通过氮相互作用调节基因型的作用
影响因子：
6.2
作者：
Chen Q;Liu Z;Wang B;Wang X;Lai J;Tian F
通讯作者：
Tian F
影响因子：
5.3
作者：
Yang W;Yoon J;Choi H;Fan Y;Chen R;An G
通讯作者：
An G
影响因子：
6.2
作者：
S. J. Snyman;bullet E Hajari;B. M. P. Watt;bullet Y Lu;bullet J C Kridl;Ó. Springer-Verlag;Berlin Heidelberg
通讯作者：
S. J. Snyman;bullet E Hajari;B. M. P. Watt;bullet Y Lu;bullet J C Kridl;Ó. Springer-Verlag;Berlin Heidelberg
影响因子：
2.5
作者：
T. Dinh;Kenta Watanabe;H. Takaragawa;Mai Nakabaru;Y. Kawamitsu
通讯作者：
T. Dinh;Kenta Watanabe;H. Takaragawa;Mai Nakabaru;Y. Kawamitsu
影响因子：
7.4
作者：
Scheible, WR;Morcuende, R;Stitt, M
通讯作者：
Stitt, M