Effects of dietary supplementation with histidine and β-alanine on blood plasma metabolome of broiler chickens at different ages.
Effects of dietary supplementation with histidine and β-alanine on blood plasma metabolome of broiler chickens at different ages.
复制标题
DOI：
10.1371/journal.pone.0277476
复制
发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Sauerwein, Helga
中科院分区：
文献类型：
--
作者：
Lackner, Julia;Hess, Vincent;Marx, Achim;Hosseini-Ghaffari, Morteza;Sauerwein, Helga
组氨酸是肉鸡的必需氨基酸，也是肌肽和鹅丝氨酸二肽的前体，但关于其在现代快速生长肉鸡中的代谢的信息很少。我们使用非靶向代谢组学来研究在添加和不添加β-丙氨酸的肉鸡日粮中使用不同标准化回肠可消化His：Lys比率所引起的代谢变化。将2204只肉鸡随机分为96个栏，每个栏23只鸡。将畜栏分为16个区组，每个区组包含一个畜栏，用于所有6个饲喂组（每组共16个畜栏）。这些饲喂组饲喂三种不同的His：Lys比率（分别为0.44、0.54和0.64），不补充和联合补充0.5% β-丙氨酸。在第35天或第54天屠宰每个饲养组一个单独随机选择的围栏中的5只随机选择的鸡，从颈部血管采集血液，并将血浆用于非靶向代谢组学分析。在这里，我们发现，高达56.0%的所有代谢物分析的年龄发生了变化，而只有1.8%的代谢物受到影响的组氨酸：赖氨酸的比例在饮食中，1.5%的β-丙氨酸补充。双因素分析和代谢途径分析表明，组氨酸：赖氨酸比例和β-丙氨酸补充之间没有相互作用。饮食中His：Lys比例的影响仅限于组氨酸代谢，且甲氨谷氨酸浓度变化较大。补充β-丙氨酸显示几种代谢途径的代谢物发生变化; 3-氨基异丁酸盐浓度增加显示与β-丙氨酸代谢唯一直接相关。补充β-丙氨酸对组氨酸代谢影响不大。这些结果表明，所使用的补充剂对His和β-丙氨酸代谢的影响或相互作用有限。相比之下，鸟类的年龄对代谢组的影响最大。
Histidine is an essential amino acid for broiler chickens and a precursor for the dipeptides carnosine and anserine, but little information is available about its metabolism in modern, fast-growing broilers. We used untargeted metabolomics to investigate the metabolic changes caused by the use of different standardized ileal digestible His:Lys ratios in broiler diets with and without β-alanine supplementation. A total of 2204 broilers were randomly divided into 96 pens of 23 birds each. The pens were divided into 16 blocks, each containing one pen for all six feeding groups (total of 16 pens per group). These feeding groups were fed three different His:Lys ratios (0.44, 0.54, and 0.64, respectively) without and with a combination of 0.5% β-alanine supplementation. Five randomly selected chickens of one single randomly selected pen per feeding group were slaughtered on day 35 or 54, blood was collected from the neck vessel, and plasma was used for untargeted metabolomic analysis. Here we show that up to 56.0% of all metabolites analyzed were altered by age, whereas only 1.8% of metabolites were affected by the His:Lys ratio in the diet, and 1.5% by β-alanine supplementation. Two-factor analysis and metabolic pathway analysis showed no interaction between the His:Lys ratio and β-alanine supplementation. The effect of the His:Lys ratio in the diet was limited to histidine metabolism with a greater change in formiminoglutamate concentration. Supplementation of β-alanine showed changes in metabolites of several metabolic pathways; increased concentrations of 3-aminoisobutyrate showed the only direct relationship to β-alanine metabolism. The supplementation of β-alanine indicated few effects on histidine metabolism. These results suggest that the supplements used had limited effects or interactions on both His and β-alanine metabolism. In contrast, the birds’ age has the strongest influence on the metabolome.
登录
查看更多内容
影响因子：
0.7
作者：
Kralik, G.;Sak-Bosnar, M.;Kralik, I
通讯作者：
Kralik, I
DOI：
10.3390/molecules19022299
发表时间：
2014-02-21
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Bellia F;Vecchio G;Rizzarelli E
通讯作者：
Rizzarelli E
影响因子：
2
作者：
Kai, Shinichi;Watanabe, Genya;Fujimura, Shinobu
通讯作者：
Fujimura, Shinobu
影响因子：
15.9
作者：
HERBERT, V;ZALUSKY, R
通讯作者：
ZALUSKY, R
影响因子：
4.4
作者：
Soglia F;Silva AK;Lião LM;Laghi L;Petracci M
通讯作者：
Petracci M