Role of LM23 in cell proliferation and apoptosis and its expression during the testis development.
Role of LM23 in cell proliferation and apoptosis and its expression during the testis development.
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LM23在细胞增殖和凋亡中的作用及其在睾丸发育过程中的表达。
DOI：
10.1038/aja.2013.43
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发表时间：
2013-05
影响因子：
2.9
通讯作者：
Qing Liu, Ya-Juan Song, Li-Jun Meng, Meng-Chun Jia
中科院分区：
文献类型：
--
作者：
Qing Liu, Ya-Juan Song, Li-Jun Meng, Meng-Chun Jia
LM23是一种在睾丸中以阶段特异性方式特异表达的基因，它具有多种功能，对生精细胞的生与死都很重要。本研究旨在进一步研究LM23在大鼠睾丸发育过程中的表达及其在体外增殖和抗细胞凋亡中的生物学功能。采用半定量逆转录聚合酶链式反应和实时荧光定量聚合酶链式反应检测LM23在不同发育阶段的睾丸组织中的表达。结果表明，LM23基因在睾丸中的表达水平在出生后呈进行性升高。用细胞计数试剂盒8(CCK8)、集落形成效率(CFE)和流式细胞仪分析LM23在细胞增殖中的作用。结果表明，LM23基因在293T细胞中的异位表达通过增加S期细胞数而显著促进细胞增殖。采用CCK8、Annexin V、碘化丙啶染色、Western blotting等方法检测LM23在细胞凋亡中的作用。结果表明，LM23对H_2O_2诱导的293T细胞凋亡具有保护作用，至少部分通过Akt/PI3K信号通路发挥保护作用。综上所述，这些结果为LM23在睾丸发育和精子发生中的作用提供了新的见解。
LM23, a gene expressed specifically in the testis in a stage-specific manner, has a diverse range of functions that are important in both the life and death of spermatogenic cells. The aim of this study was to further investigate the expression of LM23 in the developing rat testis and the biological function of LM23 in proliferation and antiapoptosis in vitro. Semiquantitative reverse transcription (RT)-PCR and real-time PCR were used to examine the expression of LM23 in testis at different developmental stages. The results suggested that LM23 mRNA levels in the testis increased progressively after birth. The role of LM23 in proliferation was analyzed with cell counting kit-8 (CCK8), colony-forming efficiency (CFE) and flow cytometry assays. The results indicated that ectopic expression of LM23 in 293T cells significantly promoted cell proliferation by increasing cell numbers in S phase. Several methods were used, including CCK8, annexin V and propidium iodide staining and western blotting, to determine the role of LM23 in apoptosis. The results showed that LM23 played a protective role in H 2 O 2-induced apoptosis of 293T cells, mediated at least in part through the Akt/PI3K signal pathway. Taken together, these results provide new insights into the role of LM23 in the development of the testes and spermatogenesis.
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DOI：
10.2741/e81
发表时间：
2010
期刊：
Frontiers in bioscience
影响因子：
--
作者：
Mei-ling Liu;Yi-Ming Cheng;M. Jia
通讯作者：
Mei-ling Liu;Yi-Ming Cheng;M. Jia
影响因子：
5.2
作者：
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影响因子：
11.2
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E. Barnes;L. Porter;J. Lenormand;R. Dellinger;D. Donoghue
DOI：
10.1016/j.bbagen.2007.07.010
发表时间：
2007-11
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Hui Wang;Pingzhang Wang;Xiao-qiong Sun;Ying Luo;Xin Wang;D. Ma;Jun Wu
通讯作者：
Hui Wang;Pingzhang Wang;Xiao-qiong Sun;Ying Luo;Xin Wang;D. Ma;Jun Wu
影响因子：
2
作者：
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通讯作者：
Ji-Hyun Kim;Yeong-In Yang;Kyung-Tae Lee;Hee-Juhn Park;Jung-Hye Choi