SUSCEPTIBILITY OF DALL SHEEP (OVIS DALLI DALLI) TO PNEUMONIA CAUSED BY PASTEURELLA HAEMOLYTICA
SUSCEPTIBILITY OF DALL SHEEP (OVIS DALLI DALLI) TO PNEUMONIA CAUSED BY PASTEURELLA HAEMOLYTICA
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多尔羊 (Ovis DALLI DALLI) 对溶血巴氏杆菌引起的肺炎的易感性
DOI：
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发表时间：
1996
影响因子：
1.3
通讯作者：
J. Lagerquist
中科院分区：
文献类型：
--
作者：
W. Foreyt;R. Silflow;J. Lagerquist
我们对Dall绵羊的易感性进行了评估，通过测定8只达尔绵羊血液中性粒细胞对溶血性巴氏杆菌细胞毒素裂解的敏感性，以及3只达尔绵羊、2只大角绵羊和2只绵羊的免疫接种试验，研究了达尔绵羊对2株溶血性巴氏杆菌引起的细菌性肺炎的抵抗力（Ovis canadensis）和2只家养绵羊，溶血性P杆菌菌落形成单位分别为3.7 × 106和2.5 × 107。来自Dall绵羊的中性粒细胞对来自溶血性巴斯德氏菌、生物型A、血清型2（A2）的家养绵羊来源的上清液的细胞毒素裂解比来自6只大角羊的中性粒细胞更敏感。该细胞毒性细菌与用于2只Dall绵羊和2只家养绵羊阴道内接种的分离株相同。接种这种细胞毒性溶血性巴斯德菌A2导致第一只Dall绵羊在接种后24小时内发生致命的纤维蛋白脓性胸膜肺炎，第二只Dall绵羊在接种后52小时被安乐死之前发生肺炎病变。该溶血性巴斯德菌A2株接种于2只绵羊后未引起呼吸道疾病。一种非细胞毒性的溶血性巴斯德菌菌株，生物型T，血清型3，4，10的家绵羊源没有导致肺炎的第三个Dall羊或两只大角羊。接种前，从所有3只Dall绵羊中获得溶血性巴斯德菌生物型T分离株，但这些分离株均无细胞毒性。尸检时，从两只肺炎Dall绵羊的肺和其他组织中分离出细胞毒性溶血性巴斯德菌A2。基于这些结果，我们得出结论，Dall羊似乎至少与大角羊一样敏感，以肺炎所造成的溶血性巴斯德菌A2的国内绵羊来源。由于体外和体内结果在本研究和其他研究中密切相关，我们相信通过额外的评价和标准化，中性粒细胞细胞毒性试验可在未来的野生绵羊致病性溶血性巴斯德菌研究中替代活体动物挑战。
We evaluated susceptibility of Dall sheep (Ovis dalli dalli) to bacterial pneumonia induced by two strains of Pasteurella haemolytica of domestic sheep origin by evaluating the sensitivity of blood neutrophils of eight Dall sheep to lysis by cytotoxins of P haemolytica, and by intratracheal inoculation of three Dall sheep, two bighorn sheep (Ovis canadensis), and two domestic sheep with 3.7 × 106 or 2.5 × 107 colony forming units of P haemolytica. Neutrophils from the Dall sheep were more sensitive to lysis by cytotoxins from supernatants of a P. haemolytica, biotype A, serotype 2 (A2), of domestic sheep origin, than were neutrophils from six bighorn sheep. This cytotoxic bacterium was the same isolate that was used for intratracheal inoculation of two Dall sheep and two domestic sheep. Inoculation of this cytotoxic P. haemolytica A2 resulted in fatal fibrinopurulent pleuropneumonia in the first Dall sheep within 24 hr of inoculation, and pneumonic lesions in the second Dall sheep before it was euthanized 52 hr after inoculation. This strain of P. haemolytica A2 did not cause respiratory disease when inoculated into two domestic sheep. A noncytotoxic strain of P. haemolytica, biotype T, serotype 3,4,10 of domestic sheep origin did not result in pneumonia in the third Dall sheep or two bighorn sheep. Prior to inoculation, P. haemolytica, biotype T isolates were obtained from all three Dall sheep, but none of these isolates was cytotoxic. At necropsy, cytotoxic P. haemolytica A2 was isolated from lungs and other tissues of the two pneumonic Dall sheep. Based on these results, we conclude that Dall sheep appear to be at least as sensitive as bighorn sheep to pneumonia caused by P. haemolytica A2 of domestic sheep origin. Because in vitro and in vivo results appear closely correlated in this and other studies, we believe with additional evaluation and standardization, neutrophil cytotoxicity tests may serve as a substitute for live animal challenges in future studies of pathogenic P. haemolytica in wild sheep.
影响因子：
2.2
作者：
KORZENIEWSKI, C;CALLEWAERT, DM
通讯作者：
CALLEWAERT, DM