Combining signals from spotted cDNA microarrays obtained at different scanning intensities
Combining signals from spotted cDNA microarrays obtained at different scanning intensities
复制标题
组合来自不同扫描强度下获得的斑点 cDNA 微阵列的信号
DOI：
10.1093/bioinformatics/btk047
复制
发表时间：
2006
期刊：
影响因子：
5.8
通讯作者：
Hochholdinger F.
中科院分区：
文献类型：
--
作者：
Piepho H.P;Keller B;Höcker N;Hochholdinger F.
Motivation:The analysis of spotted cDNA microarrays involves scanning of color signals from fluorescent dyes. A common problem is that a given scanning intensity is not usually optimal for all spotted cDNAs. Specifically, some spots may be at the saturation limit, resulting in poor separation of signals from different tissues or conditions. The problem may be addressed by multiple scans with varying scanning intensities. Multiple scanning intensities raise the question of how to combine different signals from the same spot, particularly when measurement error is not negligible.Results:This paper suggests a non-linear latent regression model for this purpose. It corrects for biases caused by the saturation limit and efficiently combines data from multiple scans. Combining multiple scans also allows reduction of technical error particularly for cDNA spots with low signal. The procedure is exemplified using cDNA expression data from maize.Availability:All methods were implemented using standard procedures available in the SAS/STAT module of the SAS System. Programming statements are available from the first author upon request.Contact:piepho@uni-hohenheim.deSupplementary information: The supplementary data are available atBioinformaticsonline.
具有方差分量的不平衡线性模型的分析
DOI：
--
发表时间：
1990
期刊：
影响因子：
--
作者：
B. Engel
通讯作者：
B. Engel
影响因子：
2.7
作者：
Hegde, P;Qi, R;Quackenbush, J
通讯作者：
Quackenbush, J
影响因子：
14.9
作者：
Romualdi, C;Trevisan, S;Lanfranchi, G
通讯作者：
Lanfranchi, G