Interactions of Volatile Anesthetics with Cholinergic, Tachykinin, and Leukotriene Mechanisms in Isolated Guinea Pig Bronchial Smooth Muscle
Interactions of Volatile Anesthetics with Cholinergic, Tachykinin, and Leukotriene Mechanisms in Isolated Guinea Pig Bronchial Smooth Muscle
复制标题
挥发性麻醉剂与胆碱能、速激肽和白三烯机制在离体豚鼠支气管平滑肌中的相互作用
DOI：
10.1097/00000539-200212000-00032
复制
发表时间：
2002
影响因子：
5.7
通讯作者：
S. Lindahl
中科院分区：
文献类型：
--
作者：
C. Wiklund;U. Lindsten;S. Lim;S. Lindahl
我们研究了七氟醚、地氟醚和氟烷对离体豚鼠支气管平滑肌的舒张作用。将环制备物安装在充满生理盐溶液的组织浴中，并连续用含5% CO2的氧气通气。电场刺激引起对河豚毒素敏感的收缩，表明神经介导的反应。这些包括阿托品敏感性胆碱能相和对神经激肽-2受体拮抗剂SR 48968敏感的非肾上腺素能非胆碱能（NANC）相。麻醉剂被添加到组织浴的充气气体中。在1.0最小肺泡麻醉剂浓度下的七氟烷和地氟烷以及在1.0-2.0最小肺泡麻醉剂浓度下的氟烷抑制电场刺激引起的胆碱能和NANC收缩。没有一种麻醉剂影响对外源性神经激肽A的反应，神经激肽A是NANC收缩的可能介质，表明NANC神经传递的连接前抑制。麻醉剂不影响对白三烯C4（LTC 4）的收缩反应的起始，LTC 4是哮喘支气管收缩的介体。然而，七氟烷和地氟烷均在LTC 4实现的稳态收缩中松弛支气管。令人惊讶的是，氟烷没有放松LTC 4收缩。关于LTC 4引起的支气管收缩，七氟烷和地氟烷是体外更有效的气道平滑肌松弛剂。
We studied relaxation of airway smooth muscle by sevoflurane, desflurane, and halothane in isolated guinea pig bronchi. Ring preparations were mounted in tissue baths filled with physiological salt solution and continuously aerated with 5% CO2 in oxygen. Electrical field stimulation induced contractions sensitive to tetrodotoxin, indicating nerve-mediated responses. These consisted of an atropine-sensitive cholinergic phase and a nonadrenergic noncholinergic (NANC) phase sensitive to SR48968, a neurokinin-2 receptor antagonist. Anesthetics were added to the gas aerating the tissue baths. Sevoflurane and desflurane at 1.0 minimum alveolar anesthetic concentration and halothane at 1.0–2.0 minimum alveolar anesthetic concentrations inhibited both cholinergic and NANC contractions to electrical field stimulation. None of the anesthetics affected responses to exogenously applied neurokinin A, a likely mediator of NANC contractions, suggesting prejunctional inhibition of NANC neurotransmission. The anesthetics did not affect the initiation of contractile responses to leukotriene C4 (LTC4), a mediator of asthmatic bronchoconstriction. However, sevoflurane and desflurane both relaxed bronchi in a steady-state contraction achieved by LTC4. Surprisingly, halothane did not relax LTC4 contractions. Concerning LTC4-elicited bronchoconstriction, sevoflurane and desflurane were more potent airway smooth muscle relaxants in vitro.
挥发性麻醉剂抑制猪气管平滑肌细胞中电压依赖性 Ca2+ 通道。
DOI：
10.1152/ajplung.1995.268.2.l187
发表时间：
1995
期刊：
The American journal of physiology
影响因子：
--
作者：
Yamakage,M;Hirshman,CA;Croxton,TL
通讯作者：
Croxton,TL
影响因子：
8.8
作者：
Banks, MI;Pearce, RA
通讯作者：
Pearce, RA
异氟烷增加烟碱乙酰胆碱受体的表观激动剂亲和力。
影响因子：
8.8
作者：
Raines,DE;Zachariah,VT
通讯作者：
Zachariah,VT