Fate map of the developing chick face: Analysis of expansion of facial primordia and establishment of the primary palate
Fate map of the developing chick face: Analysis of expansion of facial primordia and establishment of the primary palate
复制标题
发育中的雏鸡面部命运图谱：面部原基扩张分析和初级上腭的建立
作者：
I. McGonnell;J. Clarke;C. Tickle
面部原基在发育过程中会逐渐改变形状，最终导致初级腭部的形成。我们用DiI标记的方法研究了HH-阶段20和28之间的鸡胚胎四个面部原基中的每一个细胞群的扩展。成群的细胞集中在上面部的鼻窝、下面部成对的下颌原基的中线和融合部位，对整体扩张贡献最大。MSX-1转录本在高扩张区大量存在，而成纤维细胞生长因子-8转录本在与这些区域相关的外胚层中可见。许多细胞群显示出沿一个轴的优先扩展。上颌骨和下颌骨原基细胞群沿近远轴线扩展，而在额鼻团的远端，细胞群向内侧扩展。因此，突起发生在下颌和上颌原基的尖端，但在额鼻部肿块的底部。在相邻原基相邻的区域，我们发现细胞在原基之间双向移动、单向移动或根本不能检测到移动。每个原基中膨大程度最高的区域有最高比例的S相标记细胞。细胞死亡发生在一些低扩张的区域，但似乎细胞重排和嵌入也有助于成形。这些重排可能与邻近组织对原基的拉伸有关。用维甲酸处理鸡胚胎会导致初级腭裂(Tamarin等人)。[1984]J.恩布里约尔。实验莫福尔。84：105-123)。我们发现，通常有助于初级腭部形成的细胞群体的扩张减少了，但令人惊讶的是，异位细胞死亡很少。在处理过的上面部中，其他细胞群体的扩张更均匀，而不是定向的。这进一步支持了这样一种观点，即邻近组织施加的张力在上面部的整体塑造中起着重要作用。戴夫。戴恩。1998年；212：102-118。©1998 Wiley-Liss，Inc.
Developing facial primordia change shape substantially in stages leading up to primary palate formation. We investigated expansion of cell populations within each of the four facial primordia of chick embryos between HH‐stages 20 and 28, by using DiI labelling. Populations of cells centred around the nasal pits in the upper face, the midline of the paired mandibular primordia in the lower face, and at sites of fusion contribute most to overall expansion. Abundant Msx‐1 transcripts are found in regions of high expansion, and Fgf‐8 transcripts are seen in ectoderm associated with some of these regions. Many cell populations display preferential expansion along one axis. Maxillary and mandibular primordia cell populations expand along the proximodistal axis, whereas at the distal tip of the frontonasal mass, cell populations expand mediolaterally. Thus outgrowth occurs at the tips of mandibular and maxillary primordia, but at the base of the frontonasal mass. At regions where adjacent primordia abut each other, we found bidirectional movement of cells between primordia, unidirectional movement or could detect no movement at all. Regions of highest expansion in each primordium have the highest percentage of S phase labelled cells. Cell death occurs in some regions of low expansion but it seems likely that cell rearrangements and intercalations also contribute to shaping. These rearrangements could be associated with stretching of the primordia by neighbouring tissues. Treatment of chick embryos with retinoic acid causes clefts of the primary palate (Tamarin et al. [1984] J. Embryol. Exp. Morphol. 84:105–123). We found a decrease in expansion of cell populations that normally contribute to primary palate formation but surprisingly little ectopic cell death. Expansion of other cell populations in the treated upper face was more even rather than directed. This further supports the idea that tension exerted by neighbouring tissues plays a major role in global shaping of the upper face. Dev. Dyn. 1998;212:102–118. © 1998 Wiley‐Liss, Inc.
影响因子：
2.7
作者：
H. Suzuki;B. Padanilam;E. Vitale;F. Ramirez;M. Solursh
通讯作者：
H. Suzuki;B. Padanilam;E. Vitale;F. Ramirez;M. Solursh
鸡胚胎面部过程中细胞增殖的区域差异：发育过程中“合并”作用的研究。
DOI：
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发表时间：
1980
期刊：
Journal of embryology and experimental morphology
影响因子：
--
作者：
Minkoff,R
通讯作者：
Minkoff,R