A significant improvement of the efficacy of radical oxidant probes by the kinetic isotope effect.
A significant improvement of the efficacy of radical oxidant probes by the kinetic isotope effect.
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DOI：
10.1002/anie.201002228
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发表时间：
2010-08-16
影响因子：
16.6
通讯作者：
Murthy, Niren
中科院分区：
文献类型：
--
作者：
Kundu, Kousik;Knight, Sarah F.;Lee, Seungjun;Taylor, W. Robert;Murthy, Niren
自由基氧化剂的准确、灵敏检测是化学生物学领域的核心问题。[1]自由基氧化剂可以在体外用荧光无色染料检测，例如二氢乙锭（DHE）、[2]二氢罗丹明（DHR）、[3]氢菁、[4]氧化还原荧光-1、[5a]及其细胞器特异性类似物。[5b]虽然这些探针广泛用于细胞培养，但它们的准确性受到其高水平背景荧光的影响，[6]这是由光或氧气催化的自发氧化引起的。自由基氧化剂可以通过使用DHE，DHR和氢菁来检测，因为它们与细胞氧化剂（如超氧化物或羟基自由基）进行胺氧化反应[7]。然而，探针也通过与空气和光进行相同的胺氧化反应产生背景荧光;该反应通常归因于单线态氧（1 O2）的影响。[八]《中国日报》
The accurate and sensitive detection of radical oxidants is a central problem in the field of chemical biology.[1] Radical oxidants can be detected in vitro with fluorescent leucodyes such as dihydroethidium (DHE),[2] dihydrorhodamine (DHR),[3] the hydrocyanines,[4] redoxfluor-1,[5a] and their organelle-specific analogues.[5b] Although these probes are widely used in cell culture, their accuracy is compromised by their high levels of background fluorescence,[6] which is caused by their spontaneous oxidation that is catalyzed by light or oxygen. Radical oxidants can be detected by using DHE, DHR, and the hydrocyanines, as they undergo an amine oxidation reaction [7] with cellular oxidants, such as superoxide or hydroxyl radicals. However, the probes also generate background fluorescence by undergoing the same amine oxidation reaction with air and light; this reaction is often attributed to the effects of singlet oxygen (1O2).[8]
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影响因子：
7.4
作者：
Lin, Patricia W.;Myers, Loren E. S.;Ray, Laurie;Song, Shuh-Chyung;Nasr, Tala R.;Berardinelli, Andrew J.;Kundu, Kousik;Murthy, Niren;Hansen, Jason M.;Neish, Andrew S.
通讯作者：
Neish, Andrew S.
影响因子：
5.2
作者：
Baciocchi, E;Del Giacco, T;Lapi, A
通讯作者：
Lapi, A
影响因子：
15
作者：
FINKELSTEIN, E;ROSEN, GM;RAUCKMAN, EJ
通讯作者：
RAUCKMAN, EJ
作为自旋态反应性探针的细胞色素 P450 动力学同位素效应氧化叔胺
影响因子：
4.3
作者：
Li, Chunsen;Wu, Wei;Shaik, Sason
通讯作者：
Shaik, Sason
影响因子：
3.6
作者：
Dombrowski, GW;Dinnocenzo, JP;Gould, IR
通讯作者：
Gould, IR