Long-Zhi Decoction Medicated Serum Promotes Angiogenesis in Human Umbilical Vein Endothelial Cells Based on Autophagy.
Long-Zhi Decoction Medicated Serum Promotes Angiogenesis in Human Umbilical Vein Endothelial Cells Based on Autophagy.
复制标题
龙脂汤含药血清基于自噬促进人脐静脉内皮细胞血管生成
DOI：
10.1155/2018/6857398
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Zou L
中科院分区：
文献类型：
--
作者：
He Q;Liu Q;Chen Y;Meng J;Zou L
缺血性中风（IS）是一种致命的中风亚型，缺乏有效的治疗。IS后的血管生成是介导脑恢复和修复的有效反应。本课题组前期的研究表明，中药复方龙栀汤（LZD）能促进IS模型大鼠的血管生成。为了进一步研究LZD药物血清的促血管生成机制与细胞自噬之间的关联，我们评估了其对体外人脐静脉内皮细胞（HUVECs）血管生成的促进作用。采用H_2O_2诱导HUVECs损伤，观察LZD含药血清对损伤的影响。基于细胞的测定包括增殖、迁移和管形成。为了评估自噬的程度，使用透射电子显微镜来测量自噬体的数量。采用免疫荧光和Western blotting检测LC 3-II和Beclin-1的自噬相关蛋白。LZD含药血清促进HUVECs增殖、迁移和管腔形成。LZD含药血清还增加了自噬体和LC 3-II和Beclin-1的自噬蛋白表达。LZD的促血管生成和自噬活性为其临床应用提供了新的思路，并可能导致潜在的药物开发用于治疗各种血管疾病，特别是在老年人中，在未来。
Ischemic stroke (IS) is a fatal subtype of stroke that lacks effective treatments. Angiogenesis following IS is an effective response that mediates brain recovery and repair. Our previous study demonstrated that long-zhi decoction (LZD), a Chinese herbal formula, promoted angiogenesis in rats of IS model. To further investigate the association between the proangiogenic mechanism of an LZD-medicated serum and cellular autophagy, we evaluated its promotional effect on angiogenesis in human umbilical vein endothelial cells (HUVECs) in vitro. We used HUVECs subjected to H2O2 to induce injury and observed the effects of the LZD-medicated serum treatment. Cell-based assays included proliferation, migration, and tube formation. To assess the extent of autophagy, transmission electron microscopy was used to measure the number of autophagosomes. Immunofluorescence and Western blotting were performed to evaluate the autophagy-related protein of LC3-II and Beclin-1. The LZD-medicated serum promoted proliferation, migration, and tube formation in HUVECs. The LZD-medicated serum also increased the autophagosomes and the autophagic protein expressions of LC3-II and Beclin-1. The proangiogenic and autophagic activity of LZD provides new cogitations to its clinical application and may lead to potential drug development for treating various vascular diseases, especially in the elderly, in the future.
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影响因子：
9
作者：
Kumar S;Guru SK;Pathania AS;Kumar A;Bhushan S;Malik F
通讯作者：
Malik F
影响因子：
6.1
作者：
Li, Xiao-Jiang;Li, Shihua
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影响因子：
2.7
作者：
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自噬在眼部健康中的不断演变的功能：一把双刃剑。
影响因子：
9.2
作者：
Chai P;Ni H;Zhang H;Fan X
通讯作者：
Fan X
影响因子：
11.2
作者：
Gewirtz DA
通讯作者：
Gewirtz DA