Helical ordering of envelope-associated proteins and glycoproteins in respiratory syncytial virus.
Helical ordering of envelope-associated proteins and glycoproteins in respiratory syncytial virus.
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DOI：
10.15252/embj.2021109728
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发表时间：
2022-02-01
期刊：
影响因子：
--
通讯作者：
Bhella D
中科院分区：
文献类型：
--
作者：
Conley MJ;Short JM;Burns AM;Streetley J;Hutchings J;Bakker SE;Power BJ;Jaffery H;Haney J;Zanetti G;Murcia PR;Stewart M;Fearns R;Vijayakrishnan S;Bhella D
人呼吸道合胞病毒（RSV）可引起儿童和老年人严重的呼吸道疾病。在这里，使用低温电子显微镜和断层扫描结合计算机图像分析和三维重建，我们表明，有广泛的螺旋排序的包膜相关蛋白和糖蛋白的RSV丝状病毒粒子。我们计算了在病毒包膜下形成内骨架的基质蛋白（M）层的16 μ m分辨率亚断层图像平均值。这些数据定义了M-二聚体的螺旋晶格，显示了M相对于病毒包膜的取向。病毒包膜上的糖蛋白也被发现是螺旋有序的，这是一种由M层协调的特性。此外，包膜糖蛋白成对聚集，这一特征可能对作为疫苗和单克隆抗体开发的主要靶标的融合（F）糖蛋白表位的构象具有影响。我们还报告了在真实的病毒感染中，包装在RSV病毒粒子内的N-RNA环的存在。这些数据提供了对病毒体的组织及其组装机制的分子见解。人类病原体RSV的CryoEM和断层扫描分析显示丝状病毒体的形成，其表现出包膜相关基质蛋白层的螺旋排序，其进而协调病毒糖蛋白的螺旋排序和簇集。
Human respiratory syncytial virus (RSV) causes severe respiratory illness in children and the elderly. Here, using cryogenic electron microscopy and tomography combined with computational image analysis and three‐dimensional reconstruction, we show that there is extensive helical ordering of the envelope‐associated proteins and glycoproteins of RSV filamentous virions. We calculated a 16 Å resolution sub‐tomogram average of the matrix protein (M) layer that forms an endoskeleton below the viral envelope. These data define a helical lattice of M‐dimers, showing how M is oriented relative to the viral envelope. Glycoproteins that stud the viral envelope were also found to be helically ordered, a property that was coordinated by the M‐layer. Furthermore, envelope glycoproteins clustered in pairs, a feature that may have implications for the conformation of fusion (F) glycoprotein epitopes that are the principal target for vaccine and monoclonal antibody development. We also report the presence, in authentic virus infections, of N‐RNA rings packaged within RSV virions. These data provide molecular insight into the organisation of the virion and the mechanism of its assembly. CryoEM and tomography analysis of the human pathogen RSV shows the formation of filamentous virions that exhibit helical ordering of the envelope associated matrix protein layer that in turn coordinates helical ordering and clustering of viral glycoproteins.
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影响因子：
5.4
作者：
Galloux, Marie;Tarus, Bogdan;Eleouet, Jean-Francois
通讯作者：
Eleouet, Jean-Francois
影响因子：
3
作者：
Kremer, JR;Mastronarde, DN;McIntosh, JR
通讯作者：
McIntosh, JR
影响因子：
3
作者：
Crowther, RA;Henderson, R;Smith, JM
通讯作者：
Smith, JM
影响因子：
6.7
作者：
Braun, Molly R.;Deflube, Laure R.;Fearns, Rachel
通讯作者：
Fearns, Rachel
呼吸综合病毒核蛋白-RNA复合物形成左手螺旋核蛋白酶。
DOI：
10.1099/vir.0.053025-0
发表时间：
2013-08
期刊：
The Journal of general virology
影响因子：
--
作者：
Bakker SE;Duquerroy S;Galloux M;Loney C;Conner E;Eléouët JF;Rey FA;Bhella D
通讯作者：
Bhella D