Cross-species hybridisation of pig RNA to human nylon microarrays.
Cross-species hybridisation of pig RNA to human nylon microarrays.
复制标题
猪RNA与人尼龙微阵列的跨物种杂交。
DOI：
10.1186/1471-2164-3-27
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发表时间：
2002-09-27
期刊：
影响因子：
4.4
通讯作者：
McIntyre, L
中科院分区：
文献类型：
--
作者：
Moody, DE;Zou, Z;McIntyre, L
本研究的目的是探讨跨物种微阵列杂交的重复性。同种和跨种杂交之间的比较也进行了。一个单一的猪骨骼肌RNA样品和三个人的cDNA尼龙微阵列之间的九个杂交完成。在另外三个微阵列上完成两种不同量的猪RNA和人骨骼肌RNA的三次重复杂交。通过斯皮尔曼和皮尔逊相关系数以及Kappa统计量确定，猪cDNA与人微阵列的微阵列杂交的再现性较高。人和猪数据的重复杂交之间的变异性相似，表明结果的重现性在跨种属杂交中未受到影响。使用猪和人骨骼肌RNA杂交产生的数据之间的一致性很高，进一步支持使用人微阵列分析猪的基因表达。未发现剥离和重复使用尼龙微阵列的系统性影响，并且微阵列之间的变异性极小。大多数基因在跨物种微阵列杂交中产生高度可重复的数据，尽管约6%被鉴定为高度可变的。对于每个实验处理，包括至少三次重复杂交的实验设计将使得能够适当地考虑单个基因的变异性。跨物种微阵列分析的使用看起来很有前途。然而，需要额外的验证来确定跨物种杂交的特异性和结果的有效性。
The objective of this research was to investigate the reproducibility of cross-species microarray hybridisation. Comparisons between same- and cross-species hybridisations were also made. Nine hybridisations between a single pig skeletal muscle RNA sample and three human cDNA nylon microarrays were completed. Three replicate hybridisations of two different amounts of pig RNA, and of human skeletal muscle RNA were completed on three additional microarrays. Reproducibility of microarray hybridisations of pig cDNA to human microarrays was high, as determined by Spearman and Pearson correlation coefficients and a Kappa statistic. Variability among replicate hybridisations was similar for human and pig data, indicating the reproducibility of results were not compromised in cross-species hybridisations. The concordance between data generated from hybridisations using pig and human skeletal muscle RNA was high, further supporting the use of human microarrays for the analysis of gene expression in the pig. No systematic effect of stripping and re-using nylon microarrays was found, and variability across microarrays was minimal. The majority of genes generated highly reproducible data in cross-species microarray hybridisations, although approximately 6% were identified as highly variable. Experimental designs that include at least three replicate hybridisations for each experimental treatment will enable the variability of individual genes to be considered appropriately. The use of cross-species microarray analysis looks promising. However, additional validation is needed to determine the specificity of cross-species hybridisations, and the validity of results.
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影响因子：
7
作者：
Smith, TPL;Grosse, WM;Keele, JW
通讯作者：
Keele, JW
DOI：
10.1152/ajpregu.1999.276.2.r308
发表时间：
1999-02-01
影响因子：
2.8
作者：
Cros, N;Muller, J;Dechesne, CA
通讯作者：
Dechesne, CA
影响因子：
6.1
作者：
Huang, GS;Yang, SM;Liu, YC
通讯作者：
Liu, YC
影响因子：
2.7
作者：
Miller, NA;Gong, Q;LaBrie, ST
通讯作者：
LaBrie, ST
DOI：
10.1152/ajpheart.00292.2001
发表时间：
2002-02-01
影响因子：
4.8
作者：
Medhora, M;Bousamra, M;Jacobs, ER
通讯作者：
Jacobs, ER