Distribution of 2-[I]iodomelatonin binding in the brain of Mexican free-tailed bats (Tadarida brasiliensis).
Distribution of 2-[I]iodomelatonin binding in the brain of Mexican free-tailed bats (Tadarida brasiliensis).
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DOI：
10.1159/000202987
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Smotherman M
中科院分区：
文献类型：
--
作者：
Schwartz C;Bartell P;Cassone V;Smotherman M
对于许多季节性繁殖的动物来说，神经激素褪黑素是一种重要的信号，它可以区分一天中的时间和一年中的时间。到目前为止，在几乎所有被调查的哺乳动物中，在视交叉上核、中隆起和脑垂体中都发现了高密度的褪黑激素受体，而在其他大脑区域也发现了低密度的褪黑激素受体，这些区域以物种特异性的方式变化。由于受体分布的物种特异性差异与行为和生态的差异有关，因此对褪黑激素受体在脊椎动物大脑中的分布进行比较研究，有助于理解控制日常和季节行为的神经回路的功能组织。在这项研究中，我们使用2-[125I]碘化褪黑素体外放射自显影技术定位并表征了墨西哥无尾蝙蝠（Tadarida brasiliensis）大脑中的褪黑素结合位点。巴西小蝌蚪是一种夜间食虫哺乳动物，季节性迁徙，每年繁殖一次，并在季节性生殖行为中表现出有记录的性别二态性，最明显的是在求偶叫声中。在中隆起、视交叉上核和海马中发现了突出的2-[125I]碘黑色素结合，与在其他哺乳动物中观察到的相似。高密度的结合也定位于基底神经节的结构，包括尾状核、壳核和伏隔核，这一特征在鸣禽中常见，但在哺乳动物中没有。饱和度分析表明，观察到的结合位点对褪黑激素具有亲和力，这是Mel1a受体亚型的典型结合特性。我们得出结论，墨西哥无尾蝙蝠大脑中褪黑素受体的分布似乎与其他哺乳动物的生殖和昼夜节律系统以及鸣禽的基底神经节相似。
The neurohormone melatonin is an important signal for both time of day and time of year in many seasonally breeding animals. High densities of melatonin receptors have been found in the suprachiasmatic nucleus, median eminence, and the pituitary gland in almost all mammals investigated so far, and lower densities of melatonin receptors have also been localized to other brain regions varying in a species-specific fashion. Because species-specific differences in receptor distributions have been correlated with differences in behavior and ecology, a comparative study of how melatonin receptors are distributed in vertebrate brains can be useful to the understanding of the functional organization of neural circuits controlling daily and seasonal behaviors. In this study, we localized and characterized melatonin binding sites in the brain of the Mexican free-tailed bat (Tadarida brasiliensis) using in vitro autoradiography with 2-[125I]iodomelatonin. Tadarida brasiliensis is a nocturnal insectivorous mammal that seasonally migrates, reproduces once a year, and exhibits documented sexual dimorphisms in seasonal reproductive behaviors, most notably in courtship vocalizations. Prominent 2-[125I]iodomelatonin binding was found in the median eminence, suprachiasmatic nuclei, and hippocampus, similar to that observed in other mammals. High densities of binding were also localized to structures of the basal ganglia, including the caudate nucleus, putamen, and nucleus accumbens, a feature commonly observed in songbirds but not in mammals. Saturation analysis indicated that the observed binding sites had an affinity for melatonin typical of the binding properties for the Mel1a receptor subtype. We conclude that melatonin receptor distributions in the Mexican free-tailed bat brain appear to show similarities with the reproductive and circadian systems of other mammals and the basal ganglia of songbirds.
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DOI：
10.1073/pnas.96.8.4674
发表时间：
1999-04-13
影响因子：
11.1
作者：
Bentley, GE;Van't Hof, TJ;Ball, GF
通讯作者：
Ball, GF
影响因子：
3.8
作者：
CANONACO, M;TAVOLARO, R;FRANZONI, MF
通讯作者：
FRANZONI, MF
影响因子：
64.8
作者：
Brainard, MS;Doupe, AJ
通讯作者：
Doupe, AJ
影响因子：
1.7
作者：
CASSONE, VM;BROOKS, DS;KELM, TA
通讯作者：
KELM, TA
影响因子：
3.2
作者：
Bentley, GE;Ball, GF
通讯作者：
Ball, GF