Airborne polychlorinated biphenyls (PCBs) reduce telomerase activity and shorten telomere length in immortal human skin keratinocytes (HaCat).
Airborne polychlorinated biphenyls (PCBs) reduce telomerase activity and shorten telomere length in immortal human skin keratinocytes (HaCat).
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DOI：
10.1016/j.toxlet.2011.04.012
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发表时间：
2011-07-04
影响因子：
3.5
通讯作者：
Ludewig, G.
中科院分区：
文献类型：
--
作者：
Senthilkumar, P. K.;Klingelhutz, A. J.;Jacobus, J. A.;Lehmler, H.;Robertson, L. W.;Ludewig, G.
多氯联苯，一组209个单独的同源物，是普遍存在的环境污染物，并被列为可能的人类致癌物。一个主要的接触途径是吸入这些工业化合物，可能每天从市中心的空气和/或受污染建筑物的室内空气中吸入。衰老和癌变的标志是端粒长度和端粒酶活性的变化。我们假设，半挥发性多氯联苯，如那些发现在内城空气中，能够破坏端粒酶活性和改变端粒长度。为了探索这种可能性，我们将人类皮肤角质形成细胞暴露于合成的芝加哥多氯联苯空气混合物（CAM）或主要的空气中多氯联苯同系物，PCB 28或PCB 52长达48天，并确定端粒酶活性，端粒长度，细胞增殖和细胞周期分布。PCBs 28，52和CAM显着降低端粒酶活性从第18-48天。从第18天开始，PCB 52缩短了端粒长度，从第30天开始，PCB 28和CAM缩短了端粒长度。从第18天开始，所有PCBs都降低了细胞增殖;只有PCB 52使细胞周期的G 0/G1期细胞略有增加。PCB同系物对端粒酶活性的显著抑制和端粒长度的减少表明了这些化合物可能导致加速衰老和癌症的潜在机制。
PCBs, a group of 209 individual congeners, are ubiquitous environmental pollutants and classified as probable human carcinogens. One major route of exposure is by inhalation of these industrial compounds, possibly daily from inner city air and/or indoor air in contaminated buildings. Hallmarks of aging and carcinogenesis are changes in telomere length and telomerase activity. We hypothesize that semi-volatile PCBs, like those found in inner city air, are capable of disrupting telomerase activity and altering telomere length. To explore this possibility, we exposed human skin keratinocytes to a synthetic Chicago Airborne Mixture (CAM) of PCBs, or the prominent airborne PCB congeners, PCB28 or PCB52 for up to 48 days and determined telomerase activity, telomere length, cell proliferation, and cell cycle distribution. PCBs 28, 52 and CAM significantly reduced telomerase activity from days 18–48. Telomere length was shortened by PCB52 from day 18 and PCB28 and CAM from days 30 on. All PCBs decreased cell proliferation from day 18; only PCB52 produced a small increase of cells in G0/G1 of the cell cycle. This significant inhibition of telomerase activity and reduction of telomere length by PCB congeners suggest a potential mechanism by which these compounds could lead to accelerated aging and cancer.
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DOI：
10.1186/bcr553
发表时间：
2003
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Artandi SE
通讯作者：
Artandi SE
DOI：
10.1186/1476-069x-6-25
发表时间：
2007-08-31
期刊：
Environmental health : a global access science source
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.8
作者：
Henle, ES;Han, ZX;Linn, S
通讯作者：
Linn, S
影响因子：
14.9
作者：
Bailey SM;Murnane JP
通讯作者：
Murnane JP
影响因子：
4.1
作者：
Amaro, AR;Oakley, GG;Robertson, LW
通讯作者：
Robertson, LW